Effect of influenza vaccination on oxidative stress products in breath
Effect of influenza vaccination on oxidative stress products in breath
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DOI：
10.1088/1752-7155/4/2/026001
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发表时间：
2010-06-01
影响因子：
3.8
通讯作者：
Wai, James
中科院分区：
文献类型：
--
作者：
Phillips, Michael;Cataneo, Renee N.;Wai, James
病毒感染导致氧化应激增加，因此呼吸测试氧化应激生物标志物（烷烃和烷烃衍生物）可能为早期诊断提供新的工具。我们研究了33名接受流感减毒活疫苗（LAIV）治疗的正常健康人。每个受试者都是他或她自己的对照，因为他们在接种前0天进行了研究，然后在接种后的第2、7和14天进行了研究。呼气挥发性有机化合物（VOCs）由呼气收集装置收集，然后通过自动热脱附气相色谱和质谱分析。蒙特卡罗模拟技术基于c统计值（受者工作特征曲线下面积）识别感染的非随机VOC生物标志物。在LAIV治疗之后，呼吸中挥发性有机化合物的丰度发生了非随机变化。2,8 -二甲基十一烷及其他烷烃衍生物均在全天观察。保守的多变量模型在第2天识别出接种疫苗的受试者（c -统计量= 0.82，敏感性= 63.6%，特异性= 88.5%）；第7天（C-statistic = 0.94，敏感性= 88.5%，特异性= 92.3%）；第14天（C-statistic = 0.95，敏感性= 92.3%，特异性= 92.3%）。除人为污染外，在流感减毒活疫苗中未检测到呼吸中挥发性有机化合物的改变。健康人接种LAIV疫苗可引起氧化应激呼吸生物标志物的迅速和持续增加。对这些挥发性有机化合物进行呼气测试可能会识别出急性流感感染但尚未出现临床症状或疾病体征的人。
Viral infections cause increased oxidative stress, so a breath test for oxidative stress biomarkers (alkanes and alkane derivatives) might provide a new tool for early diagnosis. We studied 33 normal healthy human subjects receiving scheduled treatment with live attenuated influenza vaccine (LAIV). Each subject was his or her own control, since they were studied on day 0 prior to vaccination, and then on days 2, 7 and 14 following vaccination. Breath volatile organic compounds (VOCs) were collected with a breath collection apparatus, then analyzed by automated thermal desorption with gas chromatography and mass spectroscopy. A Monte Carlo simulation technique identified non-random VOC biomarkers of infection based on their C-statistic values (area under curve of receiver operating characteristic). Treatment with LAIV was followed by non-random changes in the abundance of breath VOCs. 2, 8-Dimethylundecane and other alkane derivatives were observed on all days. Conservative multivariate models identified vaccinated subjects on day 2 (C-statistic = 0.82, sensitivity = 63.6% and specificity = 88.5%); day 7 (C-statistic = 0.94, sensitivity = 88.5% and specificity = 92.3%); and day 14 (C-statistic = 0.95, sensitivity = 92.3% and specificity = 92.3%). The altered breath VOCs were not detected in live attenuated influenza vaccine, excluding artifactual contamination. LAIV vaccination in healthy humans elicited a prompt and sustained increase in breath biomarkers of oxidative stress. A breath test for these VOCs could potentially identify humans who are acutely infected with influenza, but who have not yet developed clinical symptoms or signs of disease.