Significance of urinary full-length megalin in patients with IgA nephropathy.
Significance of urinary full-length megalin in patients with IgA nephropathy.
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DOI：
10.1371/journal.pone.0114400
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Saito A
中科院分区：
文献类型：
--
作者：
Seki T;Asanuma K;Asao R;Nonaka K;Sasaki Y;Oliva Trejo JA;Kurosawa H;Hirayama Y;Horikoshi S;Tomino Y;Saito A
巨蛋白在近端肾小管上皮细胞的顶膜高度表达。尿全长巨蛋白（C-巨蛋白）测定与2型糖尿病中糖尿病肾病的严重程度相关。本研究旨在探讨成人伊加肾病（IgAN）患者尿C-巨蛋白水平与组织学检查结果之间的关系。从73名IgAN患者（29名男性和44名女性;平均年龄33岁）和5名膜性肾病（MN）患者中获得肾活检当天早晨排尿的尿液样本。采用牛津IgAN分类、重松分类和日本IgAN临床指南分析肾脏病理变量。采用双抗体夹心ELISA法检测尿C-巨蛋白水平。组织学分析显示，IgAN患者尿C-megalin水平与系膜细胞增多相关（OR = 1.76，95% CI：1.04-3.27，P<0.05）。 IgAN患者尿C-megalin水平与Shigematsu分型的慢性毛细血管外病变严重程度显著相关（β = 0.33，P = 0.008）。 根据日本IgAN临床指南，所有需要透析的IgAN患者的尿C-巨蛋白水平均显著高于对照组。高危组和极高危组尿C-megalin水平明显高于低危组（P<0.05）。与对照组相比，MN患者的尿C-megalin水平显著升高。尿C-巨蛋白水平与成人IgAN患者的组织学异常相关。尿C-巨蛋白可能是IgAN疾病进展的独立预测因子。此外，我们的研究结果表明，尿C-megalin是一个标志物的肾小球异常，在各种肾小球疾病以及IgA肾病。
Megalin is highly expressed at the apical membranes of proximal tubular epithelial cells. A urinary full-length megalin (C-megalin) assay is linked to the severity of diabetic nephropathy in type 2 diabetes. This study examined the relationship between levels of urinary C-megalin and histological findings in adult patients with IgA nephropathy (IgAN). Urine samples voided in the morning on the day of renal biopsy were obtained from 73 patients with IgAN (29 men and 44 women; mean age, 33 years) and 5 patients with membranous nephropathy (MN). Renal pathologic variables were analyzed using the Oxford classification of IgAN, the Shigematsu classification and the Clinical Guidelines of IgAN in Japan. The levels of urinary C-megalin were measured by sandwich ELISA. Histological analysis based on the Oxford classification revealed that the levels of urinary C-megalin were correlated with mesangial hypercellularity in IgAN patients (OR = 1.76, 95% CI: 1.04–3.27, P<0.05). There was a significant correlation between the levels of urinary C-megalin and the severity of chronic extracapillary abnormalities according to the Shigematsu classification in IgAN patients (β = 0.33, P = 0.008). The levels of urinary C-megalin were significantly higher in all risk levels of IgAN patients requiring dialysis using the Clinical Guidelines of IgAN in Japan than in the control group. The levels of urinary C-megalin were significantly higher in the high risk and very high risk grades than in the low risk grade (P<0.05). The levels of urinary C-megalin were significantly higher in MN patients compared to the control group. The levels of urinary C-megalin are associated with histological abnormalities in adult IgAN patients. There is a possibility that urinary C-megalin is an independent predictor of disease progression of IgAN. In addition, our results suggest that urinary C-megalin is a marker of glomerular abnormalities in various glomerular diseases as well as IgAN.
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DOI：
10.1152/ajprenal.00243.2004
发表时间：
2005-02-01
影响因子：
4.2
作者：
Gonzalez-Villalobos, R;Klassen, RB;Hammond, TG
通讯作者：
Hammond, TG
DOI：
10.1083/jcb.139.1.193
发表时间：
1997-10-06
期刊：
The Journal of cell biology
影响因子：
--
作者：
Mundel P;Heid HW;Mundel TM;Krüger M;Reiser J;Kriz W
通讯作者：
Kriz W
影响因子：
19.6
作者：
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影响因子：
19.6
作者：
Cattran, Daniel C.;Coppo, Rosanna;Zhang, Hong
通讯作者：
Zhang, Hong
影响因子：
13.2
作者：
Haas, M
通讯作者：
Haas, M