Bacteriophage Infection in Rod-Shaped Gram-Positive Bacteria: Evidence for a Preferential Polar Route for Phage SPP1 Entry in Bacillus subtilis
Bacteriophage Infection in Rod-Shaped Gram-Positive Bacteria: Evidence for a Preferential Polar Route for Phage SPP1 Entry in Bacillus subtilis
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DOI：
10.1128/jb.05104-11
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发表时间：
2011-09-01
影响因子：
3.2
通讯作者：
Tavares, Paulo
中科院分区：
文献类型：
--
作者：
Jakutyte, Lina;Baptista, Catarina;Tavares, Paulo
进入宿主细菌细胞是噬菌体生命周期中最不了解的步骤之一。革兰氏阴性菌和革兰氏阳性菌的不同包膜分别具有流体外膜并将厚的肽聚糖壁暴露于环境，这对噬菌体在细菌表面上的结合和（重新）分布提出了不同的挑战。这里，在空间和时间上监测噬菌体SPP 1对革兰氏阳性杆状细菌枯草芽孢杆菌的感染。我们发现，SPP 1的可逆吸附优先发生在细胞两极。这种初始结合促进了对SPP1噬菌体受体蛋白YueB的不可逆吸附，该蛋白由推定的VII型分泌系统基因簇编码。发现YueB集中在细胞两极，并沿B的侧壁沿着呈点状分布。枯草杆菌细胞在感染过程中观察SPP1 DNA进入和复制的动力学。大多数感染的DNA进入细胞两极附近并开始复制。总之，我们的研究结果表明，优先极性拓扑结构的SPP1受体的宿主细胞表面上决定的噬菌体DNA进入和随后的复制，这发生在离散的焦点的网站。
Entry into the host bacterial cell is one of the least understood steps in the life cycle of bacteriophages. The different envelopes of Gram-negative and Gram-positive bacteria, with a fluid outer membrane and exposing a thick peptidoglycan wall to the environment respectively, impose distinct challenges for bacteriophage binding and (re)distribution on the bacterial surface. Here, infection of the Gram-positive rod-shaped bacterium Bacillus subtilis by bacteriophage SPP1 was monitored in space and time. We found that SPP1 reversible adsorption occurs preferentially at the cell poles. This initial binding facilitates irreversible adsorption to the SPP1 phage receptor protein YueB, which is encoded by a putative type VII secretion system gene cluster. YueB was found to concentrate at the cell poles and to display a punctate peripheral distribution along the sidewalls of B. subtilis cells. The kinetics of SPP1 DNA entry and replication were visualized during infection. Most of the infecting phages DNA entered and initiated replication near the cell poles. Altogether, our results reveal that the preferentially polar topology of SPP1 receptors on the surface of the host cell determines the site of phage DNA entry and subsequent replication, which occurs in discrete foci.