Prostaglandin E2 adds bone to a cancellous bone site with a closed growth plate and low bone turnover in ovariectomized rats.
Prostaglandin E2 adds bone to a cancellous bone site with a closed growth plate and low bone turnover in ovariectomized rats.
复制标题
前列腺素 E2 在卵巢切除大鼠的生长板闭合且骨转换率低的松质骨部位添加骨骼。
DOI：
10.1016/8756-3282(94)90700-5
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发表时间：
1994
期刊：
影响因子：
4.1
通讯作者：
Jee,WS
中科院分区：
文献类型：
--
作者：
Ma,YF;Ke,HZ;Jee,WS
本研究的目的是确定具有闭合生长板的松质骨部位（胫骨远端干骺端，DTM）对卵巢切除术（OVX）和OVX加前列腺素E2（PGE 2）治疗的反应，并将该部位的反应与先前报告的另一部位（胫骨近端干骺端，PTM）的结果进行比较。将35只3月龄雌性SD大鼠分为5组：基础组、假去卵巢组、去卵巢+0、+1、+6 mg PGE 2/kg/d皮下注射3个月，处死前给予双荧光标记。在20 μ m厚的未脱钙DTM切片上进行松质骨组织形态计量学分析。与PTM相似，DTM显示年龄相关的骨形成减少和骨吸收增加，但不同之处在于OVX后3个月，既没有骨丢失，也没有形成终点的变化。给予OVX大鼠1 mg PGE 2/kg/d可预防大多数年龄相关变化，并维持骨形成组织形态计量学接近基础水平。用6 mg PGE 2/kg/d治疗OVX大鼠可防止年龄相关的骨变化，增加额外的骨，并通过刺激骨形成而不改变骨吸收来改善显微解剖结构。此外，PGE 2给药后，DTM（一个生长板闭合的松质骨部位）比PTM中的松质骨增加了骨形成。
The objects of this study were to determine the responses of a cancellous bone site with a closed growth plate (the distal tibial metaphysis, DTM) to ovariectomy (OVX) and OVX plus a prostaglandin E2(PGE2) treatment, and compare the site's response to previous findings reported for another site (the proximal tibial metaphysis, PTM). Thirty-five 3-month-old female Sprague-Dawley rats were divided into five groups: basal, sham-OVX, and OVX+0, +1, or +6 mg PGE2/kg/d injected subcutaneously for 3 months and given double fluorescent labels before sacrifice. Cancellous bone histomorphometric analyses were performed on 20-μm-thick undecalcified DTM sections. Similar to the PTM, the DTM showed age-related decreases in bone formation and increases in bone resorption, but it differed in that at 3 months post-OVX, there was neither bone loss nor changes in formation endpoints. Giving 1 mg PGE2/kg/d to OVX rats prevented most age-related changes and maintained the bone formation histomorphometry near basal levels. Treating OVX rats with 6 mg PGE2/kg/d prevented age-related bone changes, added extra bone, and improved microanatomical structure by stimulating bone formation without altering bone resorption. Furthermore, after PGE2administration, the DTM, a cancellous bone site with a closed growth plate, increased bone formation more than did the cancellous bone in the PTM.