Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining.
Microwave-Assisted Tissue Preparation for Rapid Fixation, Decalcification, Antigen Retrieval, Cryosectioning, and Immunostaining.
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DOI：
10.1155/2016/7076910
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发表时间：
2016
影响因子：
--
通讯作者：
Katoh K
中科院分区：
文献类型：
--
作者：
Katoh K
在固定和随后的组织化学染色过程中对组织进行微波照射显着减少了在固定和染色溶液中孵育所需的时间。最大限度地缩短固定剂中的孵育时间可减少组织形态的破坏，减少染色溶液或抗体溶液中的孵育时间可减少非特异性标记。减少染色溶液中的孵育时间也会降低背景噪音水平。微波辅助组织制备适用于组织固定、骨组织脱钙、脂肪组织处理、抗原修复以及其他组织的特殊染色。微波辅助组织固定和染色是组织学分析的有用工具。本综述描述了在组织化学研究中使用微波辐射进行几个基本程序的方案，这些技术适用于细胞生物学领域中组织固定和免疫染色的其他方案。
Microwave irradiation of tissue during fixation and subsequent histochemical staining procedures significantly reduces the time required for incubation in fixation and staining solutions. Minimizing the incubation time in fixative reduces disruption of tissue morphology, and reducing the incubation time in staining solution or antibody solution decreases nonspecific labeling. Reduction of incubation time in staining solution also decreases the level of background noise. Microwave-assisted tissue preparation is applicable for tissue fixation, decalcification of bone tissues, treatment of adipose tissues, antigen retrieval, and other special staining of tissues. Microwave-assisted tissue fixation and staining are useful tools for histological analyses. This review describes the protocols using microwave irradiation for several essential procedures in histochemical studies, and these techniques are applicable to other protocols for tissue fixation and immunostaining in the field of cell biology.