Sequence variations in estrogen receptor 1 and 2 genes and their association with egg production traits in Chinese Dagu chickens.
Sequence variations in estrogen receptor 1 and 2 genes and their association with egg production traits in Chinese Dagu chickens.
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中国大鼓鸡雌激素受体1和2基因序列变异及其与产蛋性状的关系
DOI：
10.1292/jvms.17-0014
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发表时间：
2017-05-23
期刊：
影响因子：
--
通讯作者：
Xu R
中科院分区：
文献类型：
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作者：
Niu X;Tyasi TL;Qin N;Liu D;Zhu H;Chen X;Zhang F;Yuan S;Xu R
雌激素受体α（ESR 1）和β（ESR 2）在卵泡发生中起着重要作用，因此在生殖生物学中也起着重要作用。本研究采用PCR-单链构象多态性（PCR-SSCP）和DNA测序技术，在ESR 1和ESR 2基因中发现了两个单核苷酸多态性（SNPs）。ESR 1基因第4外显子的T1101 C突变与30、43、57和66周龄的产蛋量（HHEP）和30周龄的蛋重（EW）显著相关（P <0.05）。另一个SNP，G1755 A转换导致位于ESR 2基因外显子8内的非同义替换（缬氨酸459-异亮氨酸），也与30、43、57和66周龄的HHEP（P<0.05）和30周龄的EW（P<0.05）显著相关。大部分相关产蛋性状的SNP解释了更大比例的加性方差（>1%）。此外，基于基因型的联合关联分析的结果支持了这两个SNP与所研究的性状相关的发现。综上所述，我们的研究结果表明，在ESR 1和ESR 2基因的两个序列变异可能提供有前途的遗传标记，在鸡育种的早期选择和预测的有利表型。
Estrogen receptors α (ESR1) and β (ESR2) play central roles in folliculogenesis and therefore in reproductive biology. In the present study, two single nucleotide polymorphisms (SNPs) were identified in the ESR1 and ESR2 genes using PCR-single strand conformation polymorphism (PCR-SSCP) and DNA sequencing. One of the identified SNPs, a T1101C transition located within exon 4 of the ESR1 gene, was significantly associated with hen-housed egg production (HHEP) at 30, 43, 57 and 66 weeks of age (P<0.05), and egg weight (EW) at 30 weeks (P<0.05). Another SNP, a G1755A transition leading to a non-synonymous substitution (valine 459-to-isoleucine) located within exon 8 of the ESR2 gene, was also markedly correlated with the HHEP at 30, 43, 57 and 66 weeks of age (P<0.05), and EW at 30 weeks (P<0.05). A greater proportion of the additive variance was explained by the SNPs for most of the associated egg production traits (>1%). Furthermore, the results of the combined genotype-based association analysis supported the finding that the two SNPs were associated with the traits under a study. Taken together, our findings suggest that the two sequence variations in the ESR1 and ESR2 genes may provide promising genetic markers for the early selection and prediction of advantageous phenotypes in chicken breeding.