Antioxidant capacity of phytic acid purified from rice bran - doi: 10.4025/actascitechnol.v34i4.16358
Antioxidant capacity of phytic acid purified from rice bran - doi: 10.4025/actascitechnol.v34i4.16358
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从米糠中纯化的植酸的抗氧化能力 - doi: 10.4025/actascitechnol.v34i4.16358
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发表时间：
2012
期刊：
影响因子：
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通讯作者：
E. I. Ida
中科院分区：
文献类型：
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作者：
Cristiane Canan;Fernanda Delaroza;R. Casagrande;M. Baracat;M. Shimokomaki;E. I. Ida
米糠是稻米加工业的副产品，含有高含量的植酸或植酸盐。考虑到植酸的抗氧化活性、其多种应用及其在米糠中的高浓度，本研究的目的是使用三种不同的方法评价从米糠中纯化的植酸的抗氧化能力。使用2,4,6-三吡啶-s-三嗪或FRAP（铁还原抗氧化能力）方法，对于标准或纯化的植酸没有检测到还原Fe 2+ 活性。采用BPS（红菲咯啉）法，标准植酸和米糠植酸的Fe 2+ 螯合活性取决于浓度和接触时间，观察到的IC 50 值分别为4.39 mg mL -1 和IC 50 为7.54 mg mL -1 。通过脱氧核糖法，标准植酸抑制羟自由基的IC 50 为0.70 mg mL -1 ，而米糠植酸显示出40%的最大抑制活性，与其螯合能力相关，证实了这种重要的抗氧化能力。
Rice bran is a by-product of rice processing industry, with high levels of phytic acid or phytate. Considering phytic acid antioxidant activity, its various applications and its high concentration in rice bran, this study had the objective of evaluating the antioxidant capacity of purified phytic acid from rice bran using three different methods. Using of 2,4,6-tripyridil-s-triazine or method of FRAP (Ferric Reducing Antioxidant Power) , reducing Fe 2+ activity was not detected for standard or purified phytic acid. With BPS (bathophenanthroline) method, the Fe 2+ chelator activity of standard phytic acid and rice bran phytic acid were dependent on the concentration and contact time and were observed IC 50 values of 4.39 mg mL -1 and IC 50 of 7.54 mg mL -1 , respectively. By the deoxyribose method, the standard phytic acid inhibited the hydroxyl radical with an IC 50 of 0.70 mg mL -1 while the rice bran phytic acid showed a maximum inhibitory activity of 40% associated to its chelating capacity and confirm this important antioxidant capacity.