QUANTITATION OF CHONDROITIN-4-SULFATE AND CHONDROITIN-6-SULFATE IN PATHOLOGICAL JOINT FLUID
QUANTITATION OF CHONDROITIN-4-SULFATE AND CHONDROITIN-6-SULFATE IN PATHOLOGICAL JOINT FLUID
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DOI：
10.1002/art.1780351110
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发表时间：
1992-11-01
影响因子：
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通讯作者：
MIYAZAKI, K
中科院分区：
文献类型：
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作者：
SHINMEI, M;MIYAUCHI, S;MIYAZAKI, K
目标。为探讨关节疾病与关节液中硫酸软骨素组成的相关性，对骨性关节炎(OA)、类风湿性关节炎(RA)、创伤性关节炎(TA)患者关节液中不饱和二糖异构体4-硫酸软骨素(DELTAdi-4S)和6-硫酸软骨素(DELTAdi-6S)进行了检测。病理性关节液用软骨素酶ABC消化，用高效液相色谱-荧光分光光度法检测DELTAdi-4S和DELTAdi-6S的含量。DELTAdi-4S加DELTAdi-6S在OA、RA和TA关节液中的总含量分别为71.8+/-30.0nmols/ml、55.4+/-29.3nmoles/ml和211+/-149nmoles/ml。关节液中DELTAdi-6S/DELTAdi-4S比值分别为3.81+/-0.992、1.13+/-0.527和5.75+/-2.46。两组间差异有统计学意义。这些结果有力地表明，关节液中硫酸软骨素异构体的水平和DELTAdi-6S/DELTAdi-4S比值反映了关节组织，特别是关节软骨的蛋白多糖代谢，因此，它们可以用于关节疾病的诊断和预测关节软骨的破坏。
Objective. To investigate the correlation between joint disease and the composition of chondroitin sulfate in the joint fluid, unsaturated disaccharide isomers of chondroitin 4-sulfate (DELTAdi-4S) and chondroitin 6-sulfate (DELTAdi-6S) were measured in joint fluids obtained from patients with osteoarthritis (OA), rheumatoid arthritis (RA), or traumatic arthritis (TA).Methods. These pathologic joint fluids were digested with chondroitinase ABC, and the DELTAdi-4S and DELTAdi-6S produced were determined by high performance liquid chromatography combined with fluorometry.Results. Total content of DELTAdi-4S plus DELTAdi-6S was 71.8 +/- 30.0 nmoles/ml (mean +/- SD) in OA, 55.4 +/- 29.3 nmoles/ml in RA, and 211 +/- 149 nmoles/ml in TA joint fluids. The ratio of DELTAdi-6S to DELTAdi-4S was 3.81 +/- 0.992 in OA, 1.13 +/- 0.527 in RA, and 5.75 +/- 2.46 in TA joint fluids. Differences between groups were statistically significant.Conclusion. These results strongly suggest that the levels of chondroitin sulfate isomers and the DELTAdi-6S: DELTAdi-4S ratio in joint fluid reflect the proteoglycan metabolism of joint tissues, particularly of articular cartilage; hence, they could be used to diagnose joint diseases and to predict articular cartilage destruction from such joint diseases.