NITRIC OXIDE AND ARACHIDONATE METABOLISM IN ISCHEMIA‐REPERFUSION ASSOCIATED WITH PANCREAS TRANSPLANTATION
NITRIC OXIDE AND ARACHIDONATE METABOLISM IN ISCHEMIA‐REPERFUSION ASSOCIATED WITH PANCREAS TRANSPLANTATION
复制标题
胰腺移植相关缺血再灌注中的一氧化氮和花生四烯酸代谢
DOI：
10.1097/00007890-199502000-00018
复制
发表时间：
1995
期刊：
影响因子：
6.2
通讯作者：
J. Roselló
中科院分区：
文献类型：
--
作者：
G. Hotter;D. Closa;F. Pí;N. Prats;E. Gelpí;J. Roselló
本研究探讨了类二十烷酸代谢的作用及其与大鼠胰腺移植相关缺血再灌注中一氧化氮产生的关系。将26只雄性Sprague-Dawley大鼠随机分为3组，如下：第1组，未进行手术操作的对照动物；第2组，胰腺移植，在威斯康星大学溶液中器官保存12小时后；第3组，与第2组相同，但在器官血运重建之前给予NG-硝基-L-精氨酸甲酯（一种一氧化氮合酶抑制剂）（10 mg/kg）。结果显示，移植后水肿以及胰腺组织中 6-酮前列腺素 F1α (×1.9)、血栓素 B2 (×4) 和前列腺素 E2 (×5) 水平增加。一氧化氮合酶抑制逆转了水肿和类二十烷酸产生的增加，这表明受体大鼠中类二十烷酸的产生部分是通过一氧化氮依赖性机制介导的。
The role of eicosanoid metabolism and its relationship with nitric oxide production in the ischemia-reperfusion associated with pancreas transplantation in the rat is explored in this study. Twenty-six male Sprague-Dawley rats were randomized into 3 groups, as follows: group 1, control animals not surgically manipulated; group 2, pancreas transplantation, after 12 hr of organ preservation in University of Wisconsin solution; group 3, same as group 2 but with administration of NG-nitro-L-arginine methyl ester (a nitric oxide synthase inhibitor) (10 mg/kg) before organ revascularization. The results show posttransplantation increases in edema and in 6-keto-prostaglandin F1α (×1.9), thromboxane B2 (×4), and prostaglandin E2 (×5) levels in pancreatic tissue. Nitric oxide synthase inhibition reversed the increases in edema and eicosanoid production, which suggests that eicosanoid generation in the recipient rat would be mediated, in part, through a nitric oxide-dependent mechanism.