Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.
Complete nucleotide and deduced amino acid sequence of bovine phenylethanolamine N-methyltransferase: partial amino acid homology with rat tyrosine hydroxylase.
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牛苯乙醇胺N-甲基转移酶的完整核苷酸和推导的氨基酸序列：与大鼠酪氨酸羟化酶部分氨基酸同源。
DOI：
10.1073/pnas.83.15.5454
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发表时间：
1986
影响因子：
11.1
通讯作者：
Joh,TH
中科院分区：
文献类型：
--
作者：
Baetge,EE;Suh,YH;Joh,TH
本文报道了牛苯乙醇胺n -甲基转移酶（PNMTase, EC 2.1.1.28， s -腺苷- l-蛋氨酸：苯乙醇胺n -甲基转移酶）全编码区cDNA克隆的分离。测定了cDNA的完整核苷酸序列，并推导出PNMTase的氨基酸序列。转染含有该cDNA的表达载体的培养细胞产生高水平的PNMTase酶活性。酪氨酸羟化酶[EC 1.14.16.2，酪氨酸3-单加氧酶]特异性抗体；l -酪氨酸，四氢生物碱：氧氧化还原酶（3-羟化）]，儿茶酚胺途径中的第一种酶，对体外合成的PNMTase蛋白具有惊人的亲和力。将推导出的牛PNMTase与大鼠酪氨酸羟化酶的氨基酸序列进行比较，发现PNMTase与酪氨酸羟化酶具有显著的同源性，并支持了之前蛋白质和免疫学方面的数据，表明两者在结构上是相关的。
We report here the isolation of a cDNA clone containing the full coding region of bovine phenylethanolamine N-methyltransferase (PNMTase, EC 2.1.1.28, S-adenosyl-L-methionine:phenylethanolamine N-methyltransferase). The complete nucleotide sequence of the cDNA has been determined, and the amino acid sequence of PNMTase deduced. Cultured cells transfected with an expression vector containing this cDNA produced high levels of PNMTase enzymatic activity. Antibodies specific for tyrosine hydroxylase [EC 1.14.16.2, tyrosine 3-monooxygenase; L-tyrosine, tetrahydrobiopterine: oxygen oxidoreductase (3-hydroxylating)], the first enzyme in the catecholamine pathway, possess a striking affinity for the PNMTase protein synthesized in vitro. Comparison of the deduced amino acid sequence of bovine PNMTase to rat tyrosine hydroxylase reveals that PNMTase shares significant homology with tyrosine hydroxylase and supports previous protein and immunological data suggesting that the catecholamine biosynthetic enzymes are structurally related.