Genotype Analysis of Malassezia restricta as the Major Cutaneous Flora in Patients with Atopic Dermatitis and Healthy Subjects
Genotype Analysis of Malassezia restricta as the Major Cutaneous Flora in Patients with Atopic Dermatitis and Healthy Subjects
复制标题
DOI：
10.1111/j.1348-0421.2004.tb03601.x
复制
发表时间：
2004-10
影响因子：
2.6
通讯作者：
T. Sugita;Mami Tajima;M. Amaya;R. Tsuboi;A. Nishikawa
中科院分区：
文献类型：
--
作者：
T. Sugita;Mami Tajima;M. Amaya;R. Tsuboi;A. Nishikawa
马拉色菌属的亲脂性酵母菌在人类皮肤表面定居，是特应性皮炎(AD)的加剧因素。限制分枝杆菌和球形分枝杆菌是AD患者和正常人的主要皮肤微生物区系。我们比较了13例AD患者和12例健康人皮肤表面限制分枝杆菌26S和5S rRNA基因之间的基因间隔区(IGS)的DNA序列，并与3株CBS菌株进行了比较。将IGS-1序列分为两大类，分别对应于AD患者和健康人。这些发现表明，一种特定的限制分枝杆菌基因型在AD中起着重要作用，尽管限制分枝杆菌通常在AD患者和健康受试者中定植。
Lipophilic yeasts of the genus Malassezia colonize the skin surface of humans and are an exacerbating factor in atopic dermatitis (AD). Two species, M. restricta and M. globosa are major cutaneous microflora in both AD patients and healthy subjects. We compared the DNA sequences of the intergenic spacer (IGS) region, located between the 26S and 5S rRNA genes of M. restricta colonizing the skin surfaces of 13 AD patients and 12 healthy subjects, and of three CBS stock strains as references. The IGS 1 sequences were divided into two major groups, corresponding to AD patients and healthy subjects. These findings suggest that a specific genotype of M. restricta plays a significant role in AD, although M. restricta commonly colonizes both AD patients and healthy subjects.