In Vitro Evaluations of Cytotoxicity of Eight Antidiabetic Medicinal Plants and Their Effect on GLUT4 Translocation
In Vitro Evaluations of Cytotoxicity of Eight Antidiabetic Medicinal Plants and Their Effect on GLUT4 Translocation
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DOI：
10.1155/2013/549345
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发表时间：
2013-01-01
影响因子：
--
通讯作者：
Zaid, Hilal
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文献类型：
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作者：
Kadan, Sleman;Saad, Bashar;Zaid, Hilal
尽管传统医学取得了巨大的成就，但以草药为基础的药物仍然是治疗糖尿病的常见做法。Trigonella foenum-graecum、Atriplex halimus、Olea europaea、Urtica dioica、Allium sativum、Allium cepa、Nigella sativa、肉桂肉桂在希腊-阿拉伯和伊斯兰医学中被强烈推荐用于治疗和预防糖尿病。用肝癌细胞系HepG2和大鼠L6肌细胞系细胞评价植物提取物的细胞毒性（MTT和LDH测定）。采用酶联免疫吸附试验（ELISA）对L6-GLUT4myc细胞检测植物提取物（50%乙醇水溶液）对葡萄糖转运体-4 （GLUT4）向质膜转运的影响。结果表明，肉桂决明子在浓度大于100 μ g/mL时具有细胞毒性，而其他提取物在浓度大于500 μ g/mL时均具有细胞毒性。将L6-GLUT4myc肌细胞暴露于葫芦巴（Trigonella foenum graecum）、荨麻疹（Urtica dioica）、盐柳（Atriplex halimus）和肉桂（Cinnamomum verum）的提取物中，通过MTT试验和LDH泄漏试验测量，在无细胞毒性浓度下，它们的质膜上的GLUT4显著增加。这些发现表明，这些植物的抗糖尿病特性至少部分是通过调节GLUT4易位介导的。
Despite the enormous achievements in conventional medicine, herbal-based medicines are still a common practice for the treatment of diabetes. Trigonella foenum-graecum, Atriplex halimus, Olea europaea, Urtica dioica, Allium sativum, Allium cepa, Nigella sativa, and Cinnamomum cassia are strongly recommended in the Greco-Arab and Islamic medicine for the treatment and prevention of diabetes. Cytotoxicity (MTT and LDH assays) of the plant extracts was assessed using cells from the liver hepatocellular carcinoma cell line (HepG2) and cells from the rat L6 muscle cell line. The effects of the plant extracts (50% ethanol in water) on glucose transporter-4 (GLUT4) translocation to the plasma membrane was tested in an ELISA test on L6-GLUT4myc cells. Results obtained indicate that Cinnamomon cassia is cytotoxic at concentrations higher than 100 mu g/mL, whereas all other tested extracts exhibited cytotoxic effects at concentrations higher than 500 mu g/mL. Exposing L6-GLUT4myc muscle cell to extracts from Trigonella foenum graecum, Urtica dioica, Atriplex halimus, and Cinnamomum verum led to a significant gain in GLUT4 on their plasma membranes at noncytotoxic concentrations as measured with MTT assay and the LDH leakage assay. These findings indicate that the observed anti-diabetic properties of these plants are mediated, at least partially, through regulating GLUT4 translocation.