Stress induces zinc finger immediate early genes in the rat adrenal gland
Stress induces zinc finger immediate early genes in the rat adrenal gland
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DOI：
10.1016/s0006-8993(00)02673-1
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发表时间：
2000-09-22
期刊：
影响因子：
2.9
通讯作者：
Sharp, FR
中科院分区：
文献类型：
--
作者：
Honkaniemi, J;Zhang, JS;Sharp, FR
应激刺激肾上腺皮质分泌类固醇激素，肾上腺髓质分泌促肾上腺皮质激素。本研究采用原位杂交技术，研究了辣椒素应激对大鼠肾上腺即刻早期基因NGFI-A、-B、-C、EGR-2、-3和Nurr1表达的影响。除EGR-2外，所有这些IEG均在肾上腺皮质和髓质迅速诱导。延髓IEG诱导的时间模式有显著差异。NGFI-A在应激后15min内在延髓中被诱导，NGFI-B、EGR-3和Nurr1在应激后30min被诱导，而NGFI-C在应激后2 h被诱导，令人惊讶的是，从应激15min开始，皮质网状带和束状带的糖皮质激素分泌区仅诱导了NGFI-B和Nurr1。在注射辣椒素15-30min后，所有诱导性IEG均在分泌醛固酮的球状带诱导。NGFI-A、NGFI-B和Nurr1的表达持续了6h，由于所研究的IEG在时空诱导模式上有很大的差异，它们很可能是由不同的应激诱导因素诱导的，它们在应激诱导的糖皮质激素和儿茶酚胺分泌中具有不同的靶基因和作用。(C)2000年爱思唯尔科学公司。版权所有。
The secretion of steroid hormones from the adrenal cortex as well as cathecolamines from the adrenal medulla is stimulated by stress. In this study, we studied the effect of capsaicin-induced stress on the expression of the immediate-early genes (IEGs) NGFI-A, -B, -C, egr-2, -3 and Nurr1 in the rat adrenal gland using in situ hybridization. All of these IEGs except egr-2 were rapidly induced in the adrenal cortex and medulla. The temporal patterns of the IEG induction in medulla varied significantly. NGFI-A was induced in medulla within 15 min after stress, NGFI-B, egr-3 and Nurr1 were induced by 30 min, whereas NGFI-C was induced by 2 h. Surprisingly, only NGFI-B and Nurr1 were induced in the glucocorticoid secreting regions of zonae reticularis and fasciculata of the cortex, starting 15 min after the stress. All of the inducible IEGs were induced in the aldosterone secreting zona glomerulosa 15-30 min after the capsaicin injection. NGFI-A, NGFI-B and Nurr1 expression persisted for 6 h. Since the IEGs studied had major differences in their temporospatial induction pattern, they are likely to be induced by distinct stress-elicited factors and have separate target genes and roles in stress-induced glucocorticoid and catecholamine secretion. (C) 2000 Elsevier Science BN. All rights reserved.