The effect of miR-124-3p on cell proliferation and apoptosis in bladder cancer by targeting EDNRB
The effect of miR-124-3p on cell proliferation and apoptosis in bladder cancer by targeting EDNRB
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DOI：
10.5114/aoms.2018.78743
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发表时间：
2019-09-01
影响因子：
3.8
通讯作者：
Mi, Hua
中科院分区：
文献类型：
--
作者：
Fu, Weijin;Wu, Xiaoyun;Mi, Hua
简介：内皮素受体B型（EDNRB）是miR-124- 3 p的潜在靶基因，但miR-124- 3 p与EDNRB的相关性尚未见报道。本研究旨在探讨miR-124- 3 p在膀胱癌（BC）中的作用，以及miR-124- 3 p是否通过靶向EDNRB调控细胞增殖。材料与方法：取膀胱癌组织和细胞株，采用定量RT-PCR（qRT-PCR）和Western blotting分析miR-124- 3 p和EDNRB的表达。采用双荧光素酶报告基因分析法证实miR-124- 3 p与EDNRB之间的关系。通过细胞转染实现miR-124- 3 p和EDNRB表达的操纵。MTS法、集落形成实验和流式细胞术检测细胞增殖和凋亡。结果：miR-124 - 3 p与EDNRB的表达呈负相关;在BC组织和细胞系中，miR-124- 3 p表达下调，EDNRB表达上调。观察到miR-124- 3 p靶向EDNRB并抑制其表达。结论：miR-124 - 3 p通过抑制EDNRB的表达调控BC细胞的增殖和凋亡。
Introduction: Endothelin receptor type B (EDNRB) is a potential target gene of miR-124-3p, but the association between miR-124-3p and EDNRB has not yet been reported. The aim of this study was to investigate the role of miR-124-3p in bladder cancer (BC) and to determine whether miR-124-3p regulates cell proliferation by targeting EDNRB.Material and methods: Bladder cancer tissues and cell lines were obtained in order to analyze the miR-124-3p and EDNRB expression through quantitative RT-PCR (qRT-PCR) and western blotting analysis. The dual-luciferase reporter assay was employed to confirm the relationship between miR-124-3p and EDNRB. The manipulation of miR-124-3p and EDNRB expression was achieved through cell transfection. Cell proliferation and apoptosis were evaluated by MTS assay, colony forming assay and flow cytometry. A nude mouse tumorigenicity assay was used to detect the effects of miR-124-3p in vivo.Results: There was an inverse correlation between the expression of miR-124-3p and EDNRB; miR-124-3p was down-regulated and EDNRB was up-regulated in BC tissues and cell lines. MiR-124-3p was observed to target EDNRB and suppress its expression. Other studies have suggested that the transfection of miR-124-3p mimics and EDNRB siRNA can suppress BC cell proliferation and induce cell apoptosis.Conclusions: miR-124-3p regulates the proliferation and apoptosis of BC cells by suppressing EDNRB expression.