Functional Characterization of Double-knockout Mouse Sperm Lacking SPAM1 and ACR or SPAM1 and PRSS21 in Fertilization
Functional Characterization of Double-knockout Mouse Sperm Lacking SPAM1 and ACR or SPAM1 and PRSS21 in Fertilization
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DOI：
10.1262/jrd.2011-006
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发表时间：
2012-06-01
影响因子：
1.8
通讯作者：
Baba, Tadashi
中科院分区：
文献类型：
--
作者：
Zhou, Chong;Kang, Woojin;Baba, Tadashi
哺乳动物的受精需要精子穿透卵丘到达卵母细胞。虽然精子透明质酸酶一直被认为参与穿透过程，但我们以前的工作表明，SPAM 1和HYAL 5这两种精子透明质酸酶都不是受精所必需的。在这项研究中，我们已经产生了双敲除小鼠缺乏SPAM 1和两个精子丝氨酸蛋白酶，ACR和PRSS 21之一，并表征突变精子。SPAM 1/ACR-和SPAM 1/PRSS 21-缺陷的男性是可育的，而突变小鼠的附睾精子表现出体外受精卵母细胞的能力降低。尽管正常的运动，精子穿越卵丘基质的能力更严重受损的损失SPAM 1和ACR或SPAM 1和PRSS 21比损失只有SPAM 1。此外，SPAM 1/ACR-和SPAM 1/PRSS 21-缺陷的精子比SPAM 1-缺陷的精子更丰富地积累在卵丘的表面（外缘）上。这些结果表明，ACR或PRSS 21或两者可能与SPAM 1在精子/卵丘穿透中协同作用。
Mammalian fertilization requires sperm to penetrate the cumulus to reach the oocyte. Although sperm hyaluronidase has long been believed to participate in the penetration process, our previous works revealed that neither of two sperm hyaluronidases, SPAM1 and HYAL5, are essential for fertilization. In this study, we have produced double-knockout mice lacking SPAM1 and either one of two sperm serine proteases, ACR and PRSS21, and characterized the mutant sperm. The SPAM1/ACR- and SPAM1/PRSS21-deficient males were fertile, whereas epididymal sperm of the mutant mice exhibited a reduced capacity to fertilize the oocytes in vitro. Despite normal motility, the ability of sperm to traverse the cumulus matrix was more severely impaired by the loss of SPAM1 and ACR or SPAM1 and PRSS21 than by the loss of only SPAM1. Moreover, SPAM1/ACR- and SPAM1/PRSS21-deficient sperm accumulated on the surface (outer edge) of the cumulus more abundantly than SPAM1-deficient sperm. These results suggest that ACR or PRSS21 or both may function cooperatively with SPAM1 in sperm/cumulus penetration.