Choices for Induction of Pluripotency: Recent Developments in Human Induced Pluripotent Stem Cell Reprogramming Strategies.
Choices for Induction of Pluripotency: Recent Developments in Human Induced Pluripotent Stem Cell Reprogramming Strategies.
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DOI：
10.1007/s12015-015-9622-8
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发表时间：
2016-02
影响因子：
4.8
通讯作者：
Nadif Kasri N
中科院分区：
文献类型：
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作者：
Brouwer M;Zhou H;Nadif Kasri N
从体细胞产生人类诱导多能干细胞（iPSC）的能力为再生医学带来了巨大的希望，近年来其用途广泛增加。然而，重编程效率仍然很低，并且染色体不稳定和致瘤潜力是 iPSC 使用中的问题，特别是在临床环境中。因此，重编程方法一直在开发中，以产生更安全、效率更高、质量更好的 iPSC。开发主要集中在体细胞来源、重编程因子混合物、用于引入重编程因子的递送方法以及维持生成的 iPSC 的培养条件。这篇综述讨论了这些主题的进展，并简要讨论了 iPSC 与体细胞核移植产生的人类胚胎干细胞相比的优缺点。
The ability to generate human induced pluripotent stem cells (iPSCs) from somatic cells provides tremendous promises for regenerative medicine and its use has widely increased over recent years. However, reprogramming efficiencies remain low and chromosomal instability and tumorigenic potential are concerns in the use of iPSCs, especially in clinical settings. Therefore, reprogramming methods have been under development to generate safer iPSCs with higher efficiency and better quality. Developments have mainly focused on the somatic cell source, the cocktail of reprogramming factors, the delivery method used to introduce reprogramming factors and culture conditions to maintain the generated iPSCs. This review discusses the developments on these topics and briefly discusses pros and cons of iPSCs in comparison with human embryonic stem cells generated from somatic cell nuclear transfer.