The effect of aging on nasal mucociliary clearance, beat frequency, and ultrastructure of respiratory cilia
The effect of aging on nasal mucociliary clearance, beat frequency, and ultrastructure of respiratory cilia
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DOI：
10.1164/ajrccm.163.4.9909121
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发表时间：
2001-03-01
影响因子：
24.7
通讯作者：
Tsang, KW
中科院分区：
文献类型：
--
作者：
Ho, JC;Chan, KN;Tsang, KW
老年人对下呼吸道感染的易感性增加不能完全解释。虽然受纤毛搏动和超微结构影响的粘膜纤毛清除在气道防御吸入微生物方面起着至关重要的作用，但对衰老对这些参数的影响知之甚少。我们研究了一组健康志愿者（年龄范围11至90岁）的鼻粘膜纤毛清除（NMCC）时间、纤毛搏动频率和呼吸纤毛的超微结构。用建立的光度法测定鼻纤毛上皮细胞纤毛搏动频率，用糖精试验测定NMCC时间。睫状体搏动频率（r =-0.48，p = 0.0001）和NMCC时间（r = 0.64，p < 0.001）与年龄增长相关。透射电子显微镜显示，随着年龄的增长，表现出微管紊乱和单个中心微管的受试者百分比增加（分别为p = 0.002和p = 0.005）。年龄大于40岁的受试者与年轻人相比，纤毛搏动频率明显减慢，纤毛横截面显示单小管的百分比较高，NMCC时间较长（p < 0.05），这些发现可能有助于解释老年人呼吸道感染的频繁发生。
The increased susceptibility of the elderly to lower respiratory tract infection cannot be fully explained. Although mucociliary clearance, which is affected by ciliary beating and ultrastructure, plays a crucial role in the defense of the airways against inhaled microbes, little is known of the effects of aging on these parameters. We studied the nasal mucociliary clearance (NMCC) time, ciliary beat frequency, and ultrastructure of respiratory cilia in a cohort of healthy volunteers (age range 11 to 90 yr). Ciliary beat frequency of ciliated nasal epithelial cells was obtained via an established photometric method, and NMCC time was measured with the saccharine test. There was a correlation of ciliary beat frequency (r = -0.48, p = 0.0001) and NMCC time r = 0.64, p < 0.001) with increasing age. Transmission electron microscopy revealed an increase in the percent of subjects exhibiting microtubular disarrangement and single central microtubules with aging (p = 0.002 and p = 0.005, respectively). Subjects older than 40 yr of age had significantly slower ciliary beat frequency, higher percent of ciliary cross-sections displaying single tubules, and longer NMCC time than their younger counterparts (p < 0.05), These findings may help explain the frequent occurrence of respiratory infection in the elderly.