Microbial nitrogen loss by coupled nitrification to denitrification and anammox in a permeable subterranean estuary at Gloucester Point, Virginia
Microbial nitrogen loss by coupled nitrification to denitrification and anammox in a permeable subterranean estuary at Gloucester Point, Virginia
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弗吉尼亚州格洛斯特角可渗透的地下河口中硝化、反硝化和厌氧氨氧化耦合导致的微生物氮损失
DOI：
10.1016/j.marpolbul.2021.112440
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发表时间：
2021
影响因子：
5.8
通讯作者：
Song Bongkeun
中科院分区：
文献类型：
--
作者：
Wu Jiapeng;Hong Yiguo;Wilson Stephanie J.;Song Bongkeun
在陆地和海洋之间的地下河口(STE)，氮(N)的损失过程还没有得到很好的研究。我们利用15N同位素示踪法、Q-PCR和高通量测序，揭示了美国弗吉尼亚州格洛斯特角一家STE中N丢失群落的活性、丰度和群落组成。在氧化还原梯度较大的好氧-厌氧过渡带(AATZ)中，50~60 cm深度的反硝化细菌和厌氧氨氧化细菌的活性、丰度和多样性最高，NirS-反硝化细菌和厌氧氨氧化细菌分别隶属于10个不同的类群和3个属。反硝化作用和厌氧氨氧化作用相当，估算的N损失为13.15mmolN/m−3d−1。氨氧化原核生物丰度与DO和NOx−呈正相关，表明硝化作用产生NOx−，这支持了反硝化和厌氧氨氧化的热点。总体而言，这些结果突出了N损失社区在STE中的作用。
The nitrogen (N) loss processes have not been well examined in subterranean estuaries (STEs) between land and sea. We utilized a15N isotope tracer method, q-PCR, and high-throughput sequencing to reveal the activities, abundances, and community compositions of N loss communities in a STE in Gloucester Point, Virginia, US. The highest activities, abundances and diversity of denitrifiers and anammox bacteria were detected at 50–60 cm depth in the aerobic-anaerobic transition zone (AATZ) characterized by sharp redox gradients.nirS-denitrifiers and anammox bacteria were affiliated to 10 different clusters and three genera, respectively. Denitrification and anammox played equal roles with an estimated N loss of 13.15 mmol N m−3day−1. A positive correlation between ammonia oxidizing prokaryote abundances and DO as well as NOx−suggested that nitrification produces NOx−which supports the hotspot of denitrification and anammox within the AATZ. Overall, these results highlight the roles of N loss communities in STEs.