IN-VIVO TRANSFER OF GPI-LINKED COMPLEMENT RESTRICTION FACTORS FROM ERYTHROCYTES TO THE ENDOTHELIUM
IN-VIVO TRANSFER OF GPI-LINKED COMPLEMENT RESTRICTION FACTORS FROM ERYTHROCYTES TO THE ENDOTHELIUM
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DOI：
10.1126/science.7541557
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发表时间：
1995-07-07
期刊：
影响因子：
56.9
通讯作者：
LOGAN, JS
中科院分区：
文献类型：
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作者：
KOOYMAN, DL;BYRNE, GW;LOGAN, JS
许多蛋白质与细胞膜的外层通过后修饰的糖基磷脂酰肌醇（GPI）锚结合。这种类型的蛋白质连接的功能意义尚不清楚，尽管它导致横向移动性增加，分选到细胞的顶端表面，重新插入细胞膜，并可能导致细胞信号传导。这里的证据是GPI连接的蛋白质可以在体内进行膜间转移。将转基因小鼠红细胞表面表达的GPI连接蛋白以功能性形式转移至体内内皮细胞。GPI连接的这一特征可能对将治疗性蛋白质递送至血管内皮有用。
Many proteins are associated with the outer layer of the cell membrane through a posttranslationally added glycosyl phosphatidylinositol (GPI) anchor. The functional significance of this type of protein linkage is unclear, although it results in increased lateral mobility, sorting to the apical surface of the cell, reinsertion into cell membranes, and possibly cell signaling. Here evidence is presented that GPI-linked proteins can undergo intermembrane transfer in vivo. GPI-linked proteins expressed on the surface of transgenic mouse red blood cells were transferred in a functional form to endothelial cells in vivo. This feature of GPI linkage may be potentially useful for the delivery of therapeutic proteins to vascular endothelium.