Evaluation of the Effects of Schisandra chinensis on the Myocardium of Rats with Hyperthyroid Heart Disease by Using Velocity Vector Imaging Combined with the Estimation of p53 Expression and Calmodulin Activity
Evaluation of the Effects of Schisandra chinensis on the Myocardium of Rats with Hyperthyroid Heart Disease by Using Velocity Vector Imaging Combined with the Estimation of p53 Expression and Calmodulin Activity
复制标题
速度矢量成像结合p53表达和钙调蛋白活性评估评价五味子对甲亢性心脏病大鼠心肌的影响
DOI：
10.1155/2020/5263834
复制
发表时间：
2020-07-30
影响因子：
--
通讯作者：
Jin, Chengzi
中科院分区：
文献类型：
--
作者：
Hou, Rui;Jin, Xuanshun;Jin, Chengzi
据报道五味子（SC）可以改善心肌缺血。速度矢量成像（VVI）是一种评估人类心肌功能的无创技术，但很少有关于在动物中应用的报道。在本研究中，我们旨在利用 VVI 技术评估 SC 对患有甲状腺功能亢进性心脏病 (HHD) 的 Sprague Dawley 大鼠心肌的改善作用。通过每日皮下注射左旋甲状腺素（0.5mg/kg）建立HHD模型。然后，SC组每日一次给予SC水提取物(2g/kg)，而HHD组和对照组(CON)每日给予等量的蒸馏水。所有大鼠每天给予等量的食物和水，28天后停止干预。通过超声 VVI 评估 SC 对 HHD 大鼠的疗效。通过蛋白质印迹、苏木精-伊红和马森染色以及电子显微镜评估血清总三碘甲状腺原氨酸水平、总甲状腺素水平、N末端脑钠肽前体表达、p53表达和钙调蛋白（CaM）活性。结果表明，SC通过显着降低p53表达和CaM活性，显着改善心脏的收缩速度、舒张速度、应变、收缩应变率和舒张应变率（P < 0.05），改善HHD大鼠心肌纤维化。此外，VVI 可以成为评估 HHD 大鼠心肌功能的有价值的工具。
Schisandra chinensis (SC) is reported to improve myocardial ischemia. Velocity vector imaging (VVI) is a noninvasive technique for evaluating myocardial function in humans, while few reported on the application in animals. In this study, we aimed to evaluate the improved effects of SC on the myocardium of Sprague Dawley rats having hyperthyroid heart disease (HHD) using VVI technique. HHD models were established by injecting daily with subcutaneous levothyroxine (0.5 mg/kg). Then, the SC group was administered the aqueous extract of SC (2 g/kg) once daily, while the HHD and control (CON) groups were administered the same amount of distilled water daily. All the rats were provided the same amount of food and water daily, and the intervention was stopped after 28 days. The efficacy of SC in HHD rats was evaluated by ultrasound VVI. The serum total triiodothyronine level, total thyroxine level, N-terminal pro-brain natriuretic peptide expression, p53 expression, and calmodulin (CaM) activity were assessed by western blotting, Hematoxylin-Eosin and Masson staining, and electron microscopy. The results indicated that SC significantly improved the systolic velocity, diastolic velocity, strain, systolic strain rate, and diastolic strain rate of the heart by significantly reducing p53 expression and CaM activity (P < 0.05), improving myocardial fibrosis in HHD rats. Also, VVI can be a valuable tool for the evaluation of myocardial function in HHD rats.