Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer.
Quantification of the methane concentration using anaerobic oxidation of methane coupled to extracellular electron transfer.
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DOI：
10.1016/j.biortech.2017.06.053
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发表时间：
2017-10
影响因子：
11.4
通讯作者：
Yaohuan Gao;H. Ryu;B. Rittmann;A. Hussain;Hyung-Sool Lee
中科院分区：
文献类型：
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作者：
Yaohuan Gao;H. Ryu;B. Rittmann;A. Hussain;Hyung-Sool Lee
在以甲烷为唯一电子供体的微生物电化学电池（MxC）中，生物膜阳极用含有乙酸盐的生长培养基驯化，然后用乙酸盐+甲烷，最后用单独的甲烷产生电流。而甲烷菌（Methanocorpusculum labrependid和Methanosaeta concilii）占生物膜中古菌克隆总数的82%。荧光原位杂交（FISH）成像清楚地显示了混合细菌和古菌的生物膜，这表明它们之间的互养相互作用进行甲烷厌氧氧化（AOM）的生物膜阳极。当测量持续至少50 min时，测量的累积库隆布与甲烷气体浓度在10-99.97%范围内线性相关（R2≥ 0.99）。因此，超过50 min的累积库隆布可用于定量气体样品中的甲烷浓度。
A biofilm anode acclimated with growth media containing acetate, then acetate + methane, and finally methane alone produced electrical current in a microbial electrochemical cell (MxC) fed with methane as the sole electron donor.Geobacterwas the dominant genus for the bacterial domain (93%) in the biofilm anode, while methanogens (Methanocorpusculum labreanumandMethanosaeta concilii) accounted for 82% of the total archaeal clones in the biofilm. Fluorescencein situhybridization (FISH) imaging clearly showed a biofilm of mixed bacteria and archaea, suggesting a syntrophic interaction between them for performing anaerobic oxidation of methane (AOM) in the biofilm anode. Measured cumulative coulombs were linearly correlated to the methane-gas concentration in the range of 10–99.97% (R2≥ 0.99) when the measurement was sustained for at least 50 min Thus, cumulative coulombs over 50 min could be used to quantify the methane concentration in gas samples.