Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS
Simultaneous determination of phenolic acids and flavonoids in Lycium barbarum Linnaeus by HPLC-DAD-ESI-MS
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DOI：
10.1016/j.jpba.2009.09.006
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发表时间：
2010-02-05
影响因子：
3.4
通讯作者：
Chen, B. H.
中科院分区：
文献类型：
--
作者：
Inbaraj, B. Stephen;Lu, H.;Chen, B. H.
建立了高效液相色谱-二极管阵列检测-电喷雾质谱（HPLC-DAD-ESI-MS）同时测定枸杞果实中酚酸类和黄酮类化合物的方法。用50%乙醇提取酚酸和黄酮类化合物，并使用聚合物固相萃取柱进行纯化，然后进行HPLC-DAD-ESI-MS分析。采用Vectorac C18柱，以0.5%（v/v）甲酸水溶液-乙腈-水（94：6，v/v）为流动相，梯度移动的，流速1 mL/min，柱温30 ℃，检测波长280 nm，在70 min内分离了52种酚酸和黄酮类化合物。在52种化合物中，15种酚酸类和黄酮类化合物根据吸收光谱和质谱进行了鉴定，其余37种化合物通过吸收光谱与文献报道值的比较进行了初步鉴定。内标物3-羟基苯甲酸和橙皮苷分别用于定量酚酸和黄酮类化合物。在15个阳性化合物中，槲皮素-鼠李糖-二己糖苷的质量分数最大（438.6 μ g/g），其次是槲皮素-3-O-芸香糖苷（281.3微克/克），二咖啡酰奎宁酸异构体（250.1微克/克），绿原酸（237.0微克/克），槲皮素-二-（鼠李糖己糖苷）（117.5 μ g/g），槲皮素-二（鼠李）-己糖苷（116.8 μ g/g），山奈酚-3-O-芸香糖苷（97.7 μ g/g）、异鼠李素-3-O-芸香糖苷（72.1 μ g/g）、对香豆酸（64.0 μ g/g）、咖啡酸（23.7 μ g/g）和香草酸（22.8 μ g/g）。(C)2009爱思唯尔有限公司版权所有。
A high-performance liquid chromatography-diode array detection-mass spectrometry method with electrospray ionization mode (HPLC-DAD-ESI-MS) was developed for simultaneous determination of phenolic acids and flavonoids in fruits of Lycium barbarum Linnaeus, a widely used traditional Chinese herb possessing vital biological activity. Both phenolic acids and flavonoids were extracted with 50% ethanol and purified using a polymeric solid phase extraction cartridge followed by HPLC-DAD-ESI-MS analysis. By employing a Vydac C18 column, a total of 52 phenolic acids and flavonoids were separated within 70 min using a gradient mobile phase of 0.5% (v/v) formic acid in water and acetonitrile-water (94:6, v/v) with flow rate at 1 mL/min, column temperature at 30 degrees C and detection wavelength at 280 nm. Of 52 compounds, 15 phenolic acids and flavonoids were positively identified based on both absorption and mass spectra, with the remaining 37 tentatively identified by comparison of absorption spectra with reported values in the literature. Internal standards 3-hydroxybenzoic acid and hesperidin were used for quantitation of phenolic acids and flavonoids, respectively. Among the 15 positively identified compounds, quercetin-rhamno-di-hexoside was present in largest mass fraction (438.6 mu g/g), followed by quercetin-3-O-rutinoside (281.3 mu g/g), dicaffeoylquinic acid isomers (250.1 mu g/g), chlorogenic acid (237.0 mu g/g), quercetin-di-(rhamnohexoside) (117.5 mu g/g), quercetin-di(rhamno)-hexoside (116.8 mu g/g), kaempferol-3-O-rutinoside (97.7 mu g/g), isorhamnetin-3-O-rutinoside (72.1 mu g/g), p-coumaric acid (64.0 mu g/g), caffeic acid (23.7 mu g/g) and vanillic acid (22.8 mu g/g). (C) 2009 Elsevier B.V. All rights reserved.