INTEGRATION OF THE DNA OF FILAMENTOUS BACTERIOPHAGE CFLT INTO THE CHROMOSOMAL DNA OF ITS HOST
INTEGRATION OF THE DNA OF FILAMENTOUS BACTERIOPHAGE CFLT INTO THE CHROMOSOMAL DNA OF ITS HOST
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DOI：
10.1128/jvi.61.1.60-65.1987
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发表时间：
1987-01-01
影响因子：
5.4
通讯作者：
FENG, TY
中科院分区：
文献类型：
--
作者：
KUO, TT;CHAO, YS;FENG, TY
首次证明了含有单链DNA的丝状噬菌体Cflt可以将其基因组整合到其宿主的基因组中。从分离自Cflt溶原化细胞的DNA的Southern印迹杂交分析中获得支持掺入的证据。纯化来自不同Cflt溶原化细胞的DNA，并比较整合模式。因为所有整合模式都是相同的，并且Cflt复制型DNA中只有一个片段缺失，所以似乎整合是位点特异性的。每个宿主染色体仅整合一种病毒DNA互补物。为了确定病毒DNA上的附着位点，构建了Cflt DNA上的EcoRI、XhoI、SstII和BglII的物理图谱。基于此物理图和Southern印迹杂交分析溶原DNA与这些限制性内切酶，我们证明了DNA序列从所有地区的Cflt基因组中的整合病毒序列。病毒基因组上的附着位点位于Cflt DNA上的69.2至73.8分钟处。
It was demonstrated for the first time that filamentous bacteriophage Cflt, which contains single-stranded DNA, can incorporate its genome into that of its host. Evidence in support of the incorporation was obtained from a Southern blot hybridization analysis of DNA isolated from Cflt-lysogenized cells. DNAs from different Cflt-lysogenized cells were purified, and the integration patterns were compared. Because all integration patterns were identical and only one fragment in Cflt replicative-form DNA was missing, it appears that the integration was site specific. Only one complement of viral DNA was integrated per host chromosome. To determine the attachment site on the viral DNA, the physical map of EcoRI, XhoI, SstII, and BglII on Cflt DNA was constructed. Based on this physical map and a Southern blot hybridization analysis of lysogen DNA with these restriction endonucleases, we demonstrated that DNA sequences from all regions of the Cflt genome were represented in the integrated viral sequences. The attachment site on the viral genome was located at 69.2 to 73.8 min on the Cflt DNA.