Cercopithecine Y-chromosome data provide a test of competing morphological evolutionary hypotheses.
Cercopithecine Y-chromosome data provide a test of competing morphological evolutionary hypotheses.
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DOI：
10.1016/s1055-7903(03)00024-1
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发表时间：
2003-06
影响因子：
4.1
通讯作者：
A. Tosi;T. Disotell;J. Morales;D. Melnick
中科院分区：
文献类型：
--
作者：
A. Tosi;T. Disotell;J. Morales;D. Melnick
我们在这里报告了第一次分子进化分析的结果，包括所有现存的天猿猴属的成员。对44名个体进行了睾丸特异蛋白Y染色体∼2.2kb的检测。在PAUP 4.0中，对TSPY序列进行简约分析，然后进行树比较测试，以评估现有的CECOPITE进化的形态假说。这些测试的结果表明，目前的Y染色体数据集明确支持：(1)猕猴的单系性；(2)芒果类(Cercocebus和Lopocbus)的多系性；(3)CerCopithecus的近亲；(4)将Allenopithecus和Miopithecus纳入CerCopithecini部落。文中还讨论了一些意想不到的Y染色体关系，其中包括一种暗示小球藻属复活的图案，这些小球藻目前被确定为Erythrocebus patas，Cercopithecus atheiops和Cercopithecus lhoesti。相对率测试显示，在CerCopithecini部落的许多血统中，TSPY替换率存在显著差异。由于速率差异不遵循明显的系统发育模式，因此没有使用“本地”分子钟，也没有估计这个部落的分歧日期。与之相反，类似的分析揭示了一对分类类群之间的比率异质性：猕猴和“非洲罂粟碱”。因此，通过校准这两个分支中每一个特定的TSPY时钟，计算出了部落的分歧日期。
We report here the results of the first molecular evolutionary analysis to include members of all 10 extant genera of cercopithecine monkeys. A total of 44 individuals were surveyed for ∼2.2kb of the testis-specific protein, Y-chromosome (TSPY). The TSPY sequences were subjected to parsimony analyses in PAUP 4.0, followed by tree comparison tests designed to assess existing morphological hypotheses of cercopithecine evolution. The results of these tests show that the present Y-chromosome dataset unambiguously supports: (1) monophyly of Macaca, (2) polyphyly of the mangabeys (Cercocebus and Lophocebus), (3) paraphyly of Cercopithecus, and (4) inclusion of Allenopithecus and Miopithecus in the tribe Cercopithecini. A number of unexpected Y-chromosome relationships are also discussed, including a pattern suggesting resurrection of the genus Chlorocebus for the guenons currently identified as Erythrocebus patas, Cercopithecus aethiops, and Cercopithecus lhoesti. Relative rate tests reveal significant difference in the TSPY substitution rate across numerous lineages in the tribe Cercopithecini. Because the rate differences follow no obvious phylogenetic pattern, “local” molecular clocks were not employed and divergence dates were not estimated for this tribe. In contrast, similar analysis of the Papionini reveals rate heterogeneity between a single pair of taxonomic groups: Macaca vs. the “African papionins.” Divergence dates were therefore calculated for the tribe by calibrating TSPY clocks specific to each of these two clades.