Apoptosis of HL-60 cells induced by extracts from Narcissus tazetta var. chinensis
Apoptosis of HL-60 cells induced by extracts from Narcissus tazetta var. chinensis
复制标题
水仙提取物诱导 HL-60 细胞凋亡
DOI：
10.1016/j.canlet.2005.11.023
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发表时间：
2006-10-08
期刊：
影响因子：
9.7
通讯作者：
Hu, Wei-Xin
中科院分区：
文献类型：
--
作者：
Liu, Jing;Li, Yan;Hu, Wei-Xin
中国水仙chinensis是石蒜科（Amaryllidaceae）的一员。我们发现N.塔泽塔湾结果表明，中药复方能显著降低HL-60、K562、KT 1/A3和A3 R等肿瘤细胞的存活率。ENT对NHBE和NIH 3 T3两种非癌细胞系的细胞毒作用小于对白血病细胞系的细胞毒作用。AO/EB染色和流式细胞仪检测显示，ENT可诱导HL-60细胞凋亡。ENT诱导HL-60细胞凋亡后，细胞色素c的释放和caspase-8、caspase-9、caspase-3活性的升高，提示线粒体途径和细胞死亡受体途径均参与了ENT诱导HL-60细胞凋亡的信号通路，Bax表达上调提示Bcl-2家族参与了ENT诱导HL-60细胞凋亡的调控。我们的结果表明，在HL-60细胞中，ENT可以介导凋亡活性。(c)2005爱思唯尔爱尔兰有限公司保留所有权利。
Narcissus tazetta var. chinensis is one member of the Amaryllidaceae family. We found that extracts from N. tazetta var. chinensis (ENT) strongly decreased the survival rate of the following tumor cell lines: HL-60, K562, KT1/A3, and A3R. The cytotoxic effects of ENT on non-cancer cells lines (NHBE and NIH3T3) were smaller than on leukemia cell lines. AO/EB staining and flow cytometry assays showed that ENT induced HL-60 cell apoptosis. Furthermore, the release of cytochrome c and the increase of caspase-8, -9, and -3 activities were tested after HL-60 cells were treated with ENT, which indicated that the mitochondrial pathway and cell death receptor pathway were both involved in the apoptosis signal pathways induced by ENT. Upregulation of Bax showed that the Bcl-2 family was involved in the control of apoptosis. Our results suggest that apoptosis activity can be mediated by ENT in HL-60 cells. (c) 2005 Elsevier Ireland Ltd. All rights reserved.