Quantitative diffusive wave spectroscopy in tissues
Quantitative diffusive wave spectroscopy in tissues
复制标题
组织中的定量扩散波谱
DOI：
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发表时间：
1993
期刊：
影响因子：
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通讯作者：
J. Maier
中科院分区：
文献类型：
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作者：
W. Mantulin;J. Fishkin;P. So;E. Gratton;J. Maier
高频、强度调制的光波由于高散射介质(如组织)的吸收和散射特性而衰减和相移。在距离正弦调制光源的固定距离处同时测量平均光强度、调制幅度和相移，允许从频域扫描定量确定吸收系数和散射系数的绝对值。我们的研究已经确定了对模型系统中的光学参数变化具有最高灵敏度的调制频率范围。我们测量了悬浮在高散射介质中的染料的光吸收光谱。这些光谱与在非散射介质中发现的光谱相匹配。这种频域方法提供了一种在高散射介质中执行定量光谱的简单方法。
High frequency, intensity-modulated light waves are attenuated and phase-shifted by the absorption and scattering properties of highly scattering media, such as tissue. The simultaneous measurement of the average light intensity, modulation amplitude, and phase- shift at a fixed distance from a sinusoidally modulated light source, permits a quantitative determination of the absolute values of the absorption and scattering coefficients from a frequency-domain scan. Our studies have established the range of modulation frequencies that give the highest sensitivity to changes of the optical parameters in model systems. We have measured the optical absorption spectra of dyes suspended in highly scattering media. These spectra match those found in non-scattering media. This frequency-domain approach provides a simple method to perform quantitative spectroscopy in highly scattering media.