QUANTIFICATION OF PATHOGENIC MARINE VIBRIO USING MEMBRANE-FILTER TECHNIQUE

QUANTIFICATION OF PATHOGENIC MARINE VIBRIO USING MEMBRANE-FILTER TECHNIQUE
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DOI:
10.1016/0167-7012(94)00042-6
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发表时间:
1995-02-01
影响因子:
2.2
通讯作者:
VARGASALBORES, F
VARGASALBORES, F
中科院分区:
生物学4区
文献类型:
--
作者:
HERNANDEZLOPEZ, J;GUZMANMURILLO, MA;VARGASALBORES, F

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设计了一种基于膜过滤技术的快速、简单的方法,用于检测和定量与鱼类和贝类疾病以及人类胃肠道疾病有关的海洋弧菌。通过将膜置于TCBS培养基中并在40-42 ℃下孵育,可以区分致病性和非致病性弧菌。还通过在不同盐度下培养和使用一些生化试验进行了推定鉴定。
A rapid and simple methodology based on a membrane filter technique was designed to detect and quantify marine vibrios involved in fish and shellfish diseases, as well as human gastrointestinal disorders. By placing the membrane in TCBS medium and incubating it at 40-42 degrees C, it was possible to differentiate between pathogenic and non-pathogenic vibrios. A presumptive identification was also done by growing cultures at different salinities and by using a few biochemical tests.