Platelet-Rich Plasma Stimulates Cell Proliferation and Enhances Matrix Gene Expression and Synthesis in Tenocytes From Human Rotator Cuff Tendons With Degenerative Tears
Platelet-Rich Plasma Stimulates Cell Proliferation and Enhances Matrix Gene Expression and Synthesis in Tenocytes From Human Rotator Cuff Tendons With Degenerative Tears
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DOI：
10.1177/0363546512437525
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发表时间：
2012-05-01
影响因子：
4.8
通讯作者：
Shin, Sue
中科院分区：
文献类型：
--
作者：
Jo, Chris Hyunchul;Kim, Ji Eun;Shin, Sue
背景资料：富血小板血浆（PRP）含有多种生长因子，似乎具有促进肌腱愈合的潜力，但缺乏关于其对患有退行性撕裂的肩袖肌腱中的肌腱细胞的作用的证据。假设：富血小板血浆刺激从患有退行性撕裂的肩袖肌腱中分离的肌腱细胞的细胞增殖，并增强基质基因的表达和合成。对照实验室研究：方法：Tenocytes酶分离和培养。为了评估细胞增殖，肌腱细胞与10%（体积/体积）贫血小板血浆（PPP）、钙激活的PRP以及钙和凝血酶激活的PRP一起培养14天，血小板浓度为100、200、400、800、1000、2000、4000、8000和16，000 × 10（3）/μ L。在第7天和第14天测量细胞数。为了研究基质基因的表达和合成，将细胞用PPP或PRP凝胶（10%vol/vol）以1000 × 10（3）/A μ L的血小板浓度培养14天。在第7天和第14天进行定量实时逆转录聚合酶链反应以测定I型和III型胶原、核心蛋白聚糖、腱生蛋白-C和巩膜轴的表达，并进行总胶原和糖胺聚糖（GAG）合成的测量。富血小板血浆在第7天和第14天以剂量依赖性方式显著增加细胞增殖，凝血酶的加入使增殖的平台上升。富血小板血浆在第7天显著诱导I型胶原基因表达，但在第14天不诱导，而在第7天和第14天显著促进III型胶原基因表达。III/I型胶原的比例在第7天和第14天没有变化。核心蛋白聚糖和巩膜轴蛋白的表达在第14天显著增加，而生腱蛋白-C的表达在第7天和第14天显著增加。结论：富血小板血浆可促进肌腱细胞增殖，增强基因表达，促进肌腱基质合成。临床意义：这些发现表明，PRP可作为一种有用的生物学工具，通过增强肌腱细胞的增殖和基质合成，促进肩袖撕裂的再生愈合。退化的眼泪
Background: Platelet-rich plasma (PRP) contains various growth factors and appears to have a potential to promote tendon healing, but evidence is lacking regarding its effect on human tenocytes from rotator cuff tendons with degenerative tears.Hypothesis: Platelet-rich plasma stimulates cell proliferation and enhances matrix gene expression and synthesis in tenocytes isolated from human rotator cuff tendons with degenerative tears.Study Design: Controlled laboratory study.Methods: Tenocytes were enzymatically isolated and cultured. To evaluate cell proliferation, tenocytes were cultured with 10% (vol/vol) platelet-poor plasma (PPP), PRP activated with calcium, and PRP activated with calcium and thrombin at platelet concentrations of 100, 200, 400, 800, 1000, 2000, 4000, 8000, and 16,000 x 10(3)/A mu L for 14 days. Cell number was measured at days 7 and 14. To investigate matrix gene expression and synthesis, cells were cultured with a PPP or PRP gel (10% vol/vol) at a platelet concentration of 1000 x 10(3)/A mu L for 14 days. Quantitative real-time reverse transcriptase polymerase chain reaction was performed to determine the expressions of type I and III collagen, decorin, tenascin-C, and scleraxis, and measurements of total collagen and glycosaminoglycan (GAG) synthesis were conducted at days 7 and 14.Results: Platelet-rich plasma significantly increased cell proliferation at days 7 and 14 in a dose-dependent manner, and the addition of thrombin moved up the plateau of proliferation. Platelet-rich plasma significantly induced the gene expression of type I collagen at day 7 but not at day 14, while it significantly promoted that of type III both at days 7 and 14. The ratio of type III/I collagens did not change at days 7 and 14. The expressions of decorin and scleraxis significantly increased at day 14, whereas that of tenascin-C significantly increased at days 7 and 14. Platelet-rich plasma significantly increased total collagen synthesis at days 7 and 14 and GAG synthesis at day 14.Conclusion: Platelet-rich plasma promoted cell proliferation and enhanced gene expression and the synthesis of tendon matrix in tenocytes from human rotator cuff tendons with degenerative tears.Clinical Relevance: These findings suggest that PRP might be used as a useful biological tool for regenerative healing of rotator cuff tears by enhancing the proliferation and matrix synthesis of tenocytes from tendons with degenerative tears.