FFA-1, a protein that promotes the formation of replication centers within nuclei.
FFA-1, a protein that promotes the formation of replication centers within nuclei.
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FFA-1，一种促进细胞核内复制中心形成的蛋白质。
DOI：
10.1126/science.7569932
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发表时间：
1995
期刊：
影响因子：
--
通讯作者：
Newport,J
中科院分区：
文献类型：
--
作者：
Yan,H;Newport,J
在真核细胞的细胞核中，DNA复制的起始发生在离散数目的病灶处。这些病灶的一个组分是DNA复制因子RP-A。在这里，导致RP-A与病灶结合的过程用来自Xenopuseggs的胞质组分重建。使用这种分级分离系统，鉴定并纯化了RP-A组装成病灶所需的170千道尔顿蛋白质。该蛋白质似乎是真核细胞核中启动DNA复制的焦点的组成部分。
In the nuclei of eukaryotic cells, initiation of DNA replication occurs at a discrete number of foci. One component of these foci is the DNA replication factor RP-A. Here, the process leading to the association of RP-A with foci was reconstituted with cytosolic fractions derived fromXenopuseggs. With the use of this fractionated system, a 170-kilodalton protein required for the assembly of RP-A into foci was identified and purified. The protein appears to be an integral component of the foci at which replication of DNA is initiated in eukaryotic nuclei.