Spectroscopic evidence for the preferential hydration of RNase A in glycerol-water mixtures:: Dielectric relaxation studies
Spectroscopic evidence for the preferential hydration of RNase A in glycerol-water mixtures:: Dielectric relaxation studies
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DOI：
10.1039/b008827g
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发表时间：
2001-01-01
影响因子：
3.3
通讯作者：
Stockhausen, M
中科院分区：
文献类型：
--
作者：
Betting, H;H채ckel, M;Stockhausen, M
阐明变性剂和稳定剂与蛋白质相互作用的分子机理是一个具有重要基础和实际意义的问题。在本研究中，我们提供了强有力的证据，介电弛豫研究核糖核酸酶A-甘油-水溶液的蛋白质创建自己的一个水环境中，在其附近吸引水分子从散装溶剂，从而增加甘油浓度的散装溶液。这一发现为Gekko和Timasheff（K. Gekko和S. N. Timasheff，Biochemistry，1981，20，4668;（1）K. Gekko和S. N. Timasheff，Biochemistry，1981，20，4677（2）），其报道了甘油从蛋白质结构域中的优先排除。
The elucidation of the molecular mechanism of the interaction of denaturing and stabilizing agents with proteins is a problem of eminent fundamental and practical consequences. In the present study we provide strong evidence by dielectric relaxation studies on RNase A-glycerol-water solutions that the protein creates for itself an aqueous environment in its immediate vicinity by attracting water molecules from the bulk solvent, thereby increasing the glycerol concentration of the bulk solution. This finding provides direct spectroscopic support for the thermodynamic results of Gekko and Timasheff (K. Gekko and S. N. Timasheff, Biochemistry, 1981, 20, 4668;(1) K. Gekko and S. N. Timasheff, Biochemistry, 1981, 20, 4677(2)) who reported preferential exclusion of glycerol from the protein domain.