Centromere repositioning in cucurbit species: Implication of the genomic impact from centromere activation and inactivation
Centromere repositioning in cucurbit species: Implication of the genomic impact from centromere activation and inactivation
复制标题
葫芦科物种的着丝粒重新定位：着丝粒激活和失活对基因组影响的含义
DOI：
10.1073/pnas.0904833106
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发表时间：
2009-09-01
影响因子：
11.1
通讯作者：
Jin, Weiwei
中科院分区：
文献类型：
--
作者：
Han, Yonghua;Zhang, Zhonghua;Jin, Weiwei
真核细胞染色体的着丝粒在进化过程中会移动到一个新的位置，这可能会导致染色体形态和核型的重大变化。这种着丝粒重新定位现象在哺乳动物物种中被广泛记录，并被认为在哺乳动物基因组进化中发挥着重要作用。在这里，我们报道了植物物种中的着丝粒重新定位事件。两对黄瓜(Cucumis sativus L.)常用Fosid克隆的比较荧光原位杂交定位和甜瓜(Cucumis Melo L.)染色体在进化过程中显示着丝粒位置的变化。粗线期染色体分析表明，所有四条黄瓜和甜瓜染色体的当前着丝粒都与不同的着丝粒异染色质有关。有趣的是，原始着丝粒区域着丝粒的失活与其附着的着丝粒周围异染色质的丢失或侵蚀有关。因此，葫芦科物种中着丝粒的激活和失活都与着丝粒周围异染色质的大量增减有关。
The centromere of an eukaryotic chromosome can move to a new position during evolution, which may result in a major alteration of the chromosome morphology and karyotype. This centromere repositioning phenomenon has been extensively documented in mammalian species and was implicated to play an important role in mammalian genome evolution. Here we report a centromere repositioning event in plant species. Comparative fluorescence in situ hybridization mapping using common sets of fosmid clones between two pairs of cucumber (Cucumis sativus L.) and melon (Cucumis melo L.) chromosomes revealed changes in centromere positions during evolution. Pachytene chromosome analysis revealed that the current centromeres of all four cucumber and melon chromosomes are associated with distinct pericentromeric heterochromatin. Interestingly, inactivation of a centromere in the original centromeric region was associated with a loss or erosion of its affixed pericentromeric heterochromatin. Thus, both centromere activation and inactivation in cucurbit species were associated with a gain/loss of a large amount of pericentromeric heterochromatin.