ENVELOPED DOUBLE-STRANDED DNA INSECT VIRUS WITH NOVEL STRUCTURE AND CYTOPATHOLOGY
ENVELOPED DOUBLE-STRANDED DNA INSECT VIRUS WITH NOVEL STRUCTURE AND CYTOPATHOLOGY
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DOI：
10.1073/pnas.80.24.7664
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发表时间：
1983-01-01
期刊：
影响因子：
--
通讯作者：
FEDERICI, BA
中科院分区：
文献类型：
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作者：
FEDERICI, BA
从甘蓝夜蛾Trichoplusia ni（鳞翅目;夜蛾科）幼虫中分离出一种不寻常的病毒。该病毒感染多种组织，包括脂肪体、表皮和气管基质，引起慢性、致命的疾病。病毒复制从细胞核开始，并伴有核被膜的内陷以及广泛的细胞核和细胞肥大。核被膜最终破裂并碎裂，之后病毒诱导的膜沿着沿着穿过细胞的平面并围绕其外围组装。随后，这些膜聚结，将大部分细胞（包括处于不同组装阶段的病毒颗粒和病毒粒子）分隔成一簇囊泡。囊泡解离并释放到血淋巴中，在血淋巴中大量积聚（> 108个囊泡/ml），导致血液变成不透明的白色。该病毒已从T. ni和经口和注射到T. ni和夜蛾科的其他几个物种。由该病毒产生的病毒体是大的（. apprx. 130倍400 nm），有包膜，尿囊状，具有复杂的对称性，并含有明显的线性双链DNA，分子量约为1.00. times. 108.包膜包含以六边形模式排列的亚基，其赋予负染色制备物中的病毒粒子独特的网状外观。这种病毒独特的结构和发育特性表明，它是一组以前未观察到的包膜双链DNA病毒的成员。
An unusual type of virus has been isolated from larvae of the cabbage looper, Trichoplusia ni (Lepidoptera; Noctuidae). The virus infects a variety of tissues, including fat body, epidermis and tracheal matrix, causing a chronic, fatal disease. Viral replication begins in the nucleus and is accompanied by invagination of the nuclear envelope and extensive nuclear and cellular hypertrophy. The nuclear envelope eventually ruptures and fragments, after which viral-induced membranes are assembled along planes through the cell and around its periphery. Subsequently, these membranes coalesce, partitioning most of the cell, including viroplasms and virions in various stages of assembly, among a cluster of vesicles. The vesicles dissociate and are liberated into the hemolymph where they accumulate in large numbers (> 108 vesicles/ml), causing the blood to become opaque white. The virus has been isolated from T. ni and transmitted per os and by injection to T. ni and several other species of the family Noctuidae. The virions produced by this virus are large (.apprx. 130 .times. 400 nm), enveloped, and allantoid in shape with complex symmetry and contain apparently linear, double-stranded DNA of MW of .apprx. 1.00 .times. 108. The envelope contains subunits arranged in a hexagonal pattern that impart a distinctive reticular appearance to virions in negatively stained preparations. The unique structural and developmental properties of this virus indicate that it is a member of a group of enveloped, double-stranded DNA viruses not observed previously.