Mallory bodies: isolation of hepatocellular hyalin and electrophoretic resolution of polypeptide components.
Mallory bodies: isolation of hepatocellular hyalin and electrophoretic resolution of polypeptide components.
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马洛里体：肝细胞透明蛋白的分离和多肽成分的电泳解析。
DOI：
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
S. French
中科院分区：
文献类型：
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作者：
H. Tinberg;R. J. Regan;E. Geier;G. E. Peterson;S. French
从尸检获得的人肝脏中获得纯化形式的马洛里体（MB），使用由通过Ficoll粘度屏障沉降组成的新程序。从六个肝脏中提取的制剂纯度从95%到99%不等。伴刀豆球蛋白A能强烈凝集MB。碳水化合物的存在也表明的事实，MB结合荧光标记的伴刀豆球蛋白A;没有结合观察到适当的抑制剂单糖的存在下。直接分析表明，MB含有不同量的中性己糖（每毫克蛋白质含有0.65至2.4微摩尔的葡萄糖当量），但不含唾液酸。十二烷基硫酸钠-聚丙烯酰胺凝胶电泳表明，纯化的MB含有五个主要的多肽具有表观分子量为56，000，48，500至45，000（三联体），和32，500。未检出过碘酸-希夫阳性组分。扫描电子显微镜分离的MB揭示了存在一个粗糙的，纤维状的表面，而传统的透射电子显微镜表明的丝状性质的MB。
Mallory bodies (MBs) were obtained in purified form from human liver obtained at autopsy using a new procedure consisting of sedimentation through a Ficoll viscosity barrier. Preparations from six livers ranged in purity from 95 to 99 per cent. MB preparations were autofluorescent. MBs were strongly agglutinated by Concanavalin A. The presence of carbohydrate was also indicated by the fact that MBs bound fluorescently labeled Concanavalin A; no binding was observed in the presence of appropriate inhibitor monosaccharides. Direct analysis indicated that MBs contained variable amounts of neutral hexose (0.65 to 2.4 mumoles of glucose-equivalents per milligram of protein) but no sialic acid. Sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that purified MBs contain five major polypeptides possessing apparent molecular weights of 56,000, 48,500 to 45,000 (triplet), and 32,500. Periodic acid-Schiff-positive components were not detected. Scanning electron microscopy of isolated MBs revealed the presence of a rough, fibrous surface, whereas conventional transmission electron microscopy indicated the filamentous nature of MBs.