PATTERNS OF MATRIX METALLOPROTEINASE EXPRESSION IN CYCLING ENDOMETRIUM IMPLY DIFFERENTIAL FUNCTIONS AND REGULATION BY STEROID-HORMONES
PATTERNS OF MATRIX METALLOPROTEINASE EXPRESSION IN CYCLING ENDOMETRIUM IMPLY DIFFERENTIAL FUNCTIONS AND REGULATION BY STEROID-HORMONES
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DOI：
10.1172/jci117461
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发表时间：
1994-09-01
影响因子：
15.9
通讯作者：
OSTEEN, KG
中科院分区：
文献类型：
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作者：
RODGERS, WH;MATRISIAN, LM;OSTEEN, KG
基质金属蛋白酶是一个受到高度调控的酶家族，它们一起可以降解细胞外基质的大多数成分。这些蛋白质在涉及组织重塑的正常和病理过程中具有活性；然而，人们对它们的合成位点和具体作用知之甚少。利用原位杂交，我们确定了生殖周期期间子宫内膜中基质金属蛋白酶和金属蛋白酶组织抑制剂-1（一种基质金属蛋白酶抑制剂）的细胞分布。在月经子宫内膜中检测到所有金属蛋白酶的 mRNA，但组织分布不同。 matrilysin 的 mRNA 定位于上皮细胞，而其他的 mRNA 则在基质细胞中检测到。在整个周期中仅检测到 72-kD 明胶酶和金属蛋白酶组织抑制剂-1 的转录本。 Stromelysin-2 和 92-kD 明胶酶的转录本仅在分泌晚期和月经子宫内膜中检测到，而 matrilysin、72-kD 明胶酶和 Stromelysin-3 的转录本也在增殖期子宫内膜中一致检测到。这些数据表明基质金属蛋白酶以细胞类型、组织和生殖周期特异性模式表达，与类固醇激素的调节一致，并且在生殖周期期间子宫内膜中发生的复杂组织生长和重塑过程中具有特定作用。
Matrix metalloproteinases are a highly regulated family of enzymes, that together can degrade most components of the extracellular matrix. These proteins are active in normal and pathological processes involving tissue remodeling; however, their sites of synthesis and specific roles are poorly understood. Using in situ hybridization, we determined cellular distributions of matrix metalloproteinases and tissue inhibitor of metalloproteinase-1, an inhibitor of matrix metalloproteinases, in endometrium during the reproductive cycle. The mRNAs for all the metalloproteinases were detected in menstrual endometrium, but with different tissue distributions. The mRNA for matrilysin was localized to epithelium, while the others were detected in stromal cells. Only the transcripts for the 72-kD gelatinase and tissue inhibitor of metalloproteinases-1 were detected throughout the cycle. Transcripts for stromelysin-2 and the 92-kD gelatinase were only detected in late secretory and menstrual endometrium, while those for matrilysin, the 72-kD gelatinase, and stromelysin-3 were also consistently detected in proliferative endometrium. These data indicate that matrix metalloproteinases are expressed in cell-type, tissue, and reproductive cycle-specific patterns, consistent with regulation by steroid hormones, and with specific roles in the complex tissue growth and remodeling processes occurring in the endometrium during the reproductive cycle.