PHYTOMITOGEN FROM WISTARIA-FLORIBUNDA SEEDS AND ITS INTERACTION WITH HUMAN PERIPHERAL LYMPHOCYTES
PHYTOMITOGEN FROM WISTARIA-FLORIBUNDA SEEDS AND ITS INTERACTION WITH HUMAN PERIPHERAL LYMPHOCYTES
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DOI：
10.1021/bi00800a016
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发表时间：
1971-01-01
期刊：
影响因子：
2.9
通讯作者：
OSAWA, T
中科院分区：
文献类型：
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作者：
TOYOSHIMA, S;AKIYAMA, Y;OSAWA, T
丰岛智秋山幸雄中野平。f Akira Tonomura，J和Toshiaki Osawa §摘要：从花旗参种子中纯化了一种糖蛋白，它对人外周淋巴细胞有强烈的促有丝分裂作用，但具有相对较弱的血细胞凝集和白细胞凝集活性。通过超速离心分析和圆盘电泳，该制剂是均匀的，i2 o，w值为4.5 S。这种纯化的丝裂原含有11.4%的碳水化合物，其中甘露糖（5.8%）和葡萄糖胺（3.5%）是主要的糖，还有少量的阿拉伯糖、岩藻糖和木糖。有丝分裂原刺激培养物的形态学研究表明，在72小时约70%的细胞群体已被转化的纯化的有丝分裂原。在糖对[3 H]胸苷掺入的抑制试验中，纯化的W. floriflora有丝分裂原以及其他三种非特异性有丝分裂原（concanava，
Satoshi Toyoshima, Yukio Akiyama, Kohei Nakano. f Akira Tonomura, J and Toshiaki Osawa § abstract: A glycoprotein, which is strongly mitogenic against human peripheral lymphocytes and has relatively weak hemagglutinating and leucoagglutinating activities, has been purified from the seeds of Wistaria floribunda. The prepara-tion was homogeneous by ultracentrifugal analysis and disc electrophoresis, and had a i2o, w value of 4.5 S. This purified mitogen was found to contain 11.4% carbohydrate in which mannose(5.8%) and glucosamine (3.5%) were the predomi-nant sugars, with smaller amounts of arabinose, fucose, and xylose. Morphological studies on mitogen-stimulated cultures indicated that at 72 hr about 70% of the cell population had been transformed by the purified mitogen. In the inhibition assays of [3H] thymidine incorporation by sugars, the stimula-tory activity of the purified W. floribunda mitogen as well as those of the other three nonspecific mitogens (concanava-