Genomic organization of δ-guaiene synthase genes in Aquilaria crassna and its possible use for the identification of Aquilaria species
Genomic organization of δ-guaiene synthase genes in Aquilaria crassna and its possible use for the identification of Aquilaria species
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DOI：
10.1007/s11418-011-0529-7
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发表时间：
2011-07-01
影响因子：
3.3
通讯作者：
Ito, Michiho
中科院分区：
文献类型：
--
作者：
Kumeta, Yukie;Ito, Michiho
沉香属植物的部分，称为沉香，已被用作药物和香。沉香含有多种倍半萜，一项使用厚叶沉香培养细胞的研究表明，用茉莉酸甲酯处理可诱导倍半萜（α-愈创木烯、α-蛇麻烯和δ-愈创木烯）的产生，从而导致δ-愈创木烯脱氢酶的克隆。本研究采用基因组结构分析和Southern杂交技术，对A.为了检查沉香属植物中δ-愈创木烯脱氢酶的基因组背景，进行了crassna的基因组测序。基因组克隆和测序显示5种类型的序列在推定的δ-愈创木烯脱氢酶共享超过96%的同一性，在外显子区域，这些酶属于III类TPS亚家族的7个外显子和6个内含子。Southern杂交结果表明，在A.粗鲁。将A. crassna和A.用δ-愈创木烯合酶cDNA片段制成的探针对中国对虾进行杂交，结果导致这两个物种的带型不同。用δ-愈创木烯合酶cDNA探针进行限制性片段长度多态性分析，有可能鉴别沉香属物种。
The resinous portions of Aquilaria plants, called agarwood, have been used as medicines and incenses. Agarwood contains a great variety of sesquiterpenes, and a study using cultured cells of Aquilaria crassna showed that the production of sesquiterpenes (alpha-guaiene, alpha-humulene, and delta-guaiene) was induced by treatment with methyl jasmonate, which led to the cloning of delta-guaiene synthases. In the present study, analyses of genomic organization and Southern blotting of delta-guaiene synthase in A. crassna were performed in order to examine the genomic background of delta-guaiene synthases in Aquilaria plants. Genomic cloning and sequencing revealed five types of sequence in putative delta-guaiene synthases sharing more than 96% identity in exon regions, and that these enzymes belonged to the class III TPS subfamily with seven exons and six introns. Furthermore, Southern blotting revealed that at least five copies of delta-guaiene synthase existed in A. crassna. The hybridization of digested DNA of A. crassna and A. sinensis with probes made with a delta-guaiene synthase cDNA fragment resulted in different banding patterns for these two species. It may be possible to identify Aquilaria species by restriction fragment length polymorphism analyses with delta-guaiene synthase cDNA probes.