DANSYLCADAVERINE AND CYTOCHALASIN-D ENHANCE ROTAVIRUS INFECTION OF MURINE L-CELLS
DANSYLCADAVERINE AND CYTOCHALASIN-D ENHANCE ROTAVIRUS INFECTION OF MURINE L-CELLS
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DOI：
10.1006/viro.1995.1500
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发表时间：
1995-10-01
期刊：
影响因子：
3.7
通讯作者：
UPADHYAYULA, U
中科院分区：
文献类型：
--
作者：
BASS, DM;BAYLOR, M;UPADHYAYULA, U
虽然鼠L细胞结合和内化轮状病毒以及容许细胞系，L细胞基本上是非容许的轮状病毒。在非允许的细胞系如L细胞中，内化的轮状病毒不能脱壳并保持为感染性的双壳颗粒。感染循环中的这种阻断可以通过将病毒颗粒直接脂质转染到L细胞胞质中来克服。我们假设，在L细胞内的内化轮状病毒颗粒被隔离在内吞途径，不能启动感染。在用恒河猴轮状病毒感染L细胞之前，用各种内吞抑制剂预处理L细胞。虽然抑制核内体酸化的药物对轮状病毒感染没有影响，但两种潜在的囊泡转运直接抑制剂丹磺尸胺和细胞松弛素D可增强轮状病毒对L细胞的感染5- 10倍。所有药物，包括内吞作用抑制剂和溶酶体营养剂，均显著降低了血清1型呼肠孤病毒对L细胞的感染，已知血清1型呼肠孤病毒通过内吞途径感染L细胞。时间进程研究表明，药物是有效的，促进轮状病毒感染的L细胞，只有在感染的早期阶段。预处理的L细胞与丹磺尸胺显着减少的数量完整的，双壳轮状病毒颗粒内的细胞隔离。内吞作用的抑制可以通过允许增加的附着轮状病毒病毒粒子的比例通过可能是直接膜穿透的生产性途径进入细胞来增加轮状病毒感染L细胞的效率。(C)出版社：Academic Press
Although murine L cells bind and internalize rotavirus as well as permissive cell lines, L cells are essentially nonpermissive for rotaviruses. In nonpermissive cell lines such as L cells, internalized rotavirus fails to uncoat and remains as infectious, double-shelled particles. This block in the infectious cycle can be overcome by direct lipofection of viral particles into the L cell cytoplasm. We hypothesized that the internalized rotavirus particles within L cells are sequestered in the endocytic pathway and are unable to initiate infection. L cells were pretreated with a variety of inhibitors of endocytosis prior to infection with rhesus rotavirus. While agents which inhibit acidification of endosomes had no effect on rotavirus infection, two potential direct inhibitors of vesicular transport, dansylcadaverine and cytochalasin D, enhanced rotavirus infection of L cells 5- to 10-fold. All of the drugs, including both inhibitors of endocytosis and lysosomotrophic agents, significantly reduced infection of L cells by serotype 1 reovirus which is known to infect L cells by the endocytic pathway. Time course studies demonstrated that the drugs were effective in promoting rotavirus infection of L cells in only the early phases of infection. Pretreatment of L cells with dansylcadaverine significantly decreased the number of intact, double-shelled rotavirus particles sequestered within the cells. Inhibition of endocytosis may increase the efficiency of infection of L cells by rotavirus by allowing an increased proportion of attached rotavirus virions to enter cells by a productive route which is probably direct membrane penetration. (C) 1995 Academic Press, Inc.