Isolation and characterization of four developmentally regulated cathepsin B-like cysteine protease genes from the nematode Caenorhabditis elegans
Isolation and characterization of four developmentally regulated cathepsin B-like cysteine protease genes from the nematode Caenorhabditis elegans
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DOI：
10.1089/dna.1996.15.75
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发表时间：
1996-01-01
影响因子：
3.1
通讯作者：
Johnstone, IL
中科院分区：
文献类型：
--
作者：
Larminie, CGC;Johnstone, IL
组织蛋白酶B半胱氨酸蛋白酶在真核生物中参与多种生物学过程。我们从线虫中分离并鉴定了四个不同的组织蛋白酶B样基因。这是首次在非寄生后生动物中发现的组织蛋白酶B样多基因家族。内含子的数量和大小。预测的氨基酸序列高度分化，系统发育分析表明该多基因家族在C.线虫组织蛋白酶B样基因的差异与脊椎动物和线虫组织蛋白酶B样基因之间的差异一样大。此外，这里描述的四个基因中的每一个在秀丽隐杆线虫发育过程中显示出不同的表达时间模式。
Cathepsin B cysteine protease enzymes have been shown to be involved in a variety of different biological processes in eukaryotes, We have isolated and characterized four distinct cathepsin B-like genes from the genetically tractable nematode, Caenorhabditis elegans, This is the first reported finding of a cathepsin B-like multigene family within a nonparasitic metazoan, The four genes possess distinct genomic architectures, with variations in the position, number, and size of introns. The predicted amino acid sequences of the four genes are highly diverged, Phylogenetic analysis indicates the divergence of this multigene family within C. elegans is as great as the interspecies divergence between the vertebrates and nematode cathepsin B-like genes. In addition, each of the four genes described here shows a distinct temporal pattern of expression during C, elegans development.