The nature of the RNA products synthesized in vitro by subviral components of visicular stomatitis virus.
The nature of the RNA products synthesized in vitro by subviral components of visicular stomatitis virus.
复制标题
内脏性口炎病毒亚病毒成分体外合成的 RNA 产物的性质。
DOI：
10.1016/0042-6822(76)90108-2
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发表时间：
1976
期刊：
影响因子：
3.7
通讯作者：
A. Banerjee
中科院分区：
文献类型：
--
作者：
G. Abraham;A. Banerjee
在低盐（0.4MNaCl）或高盐（0.8MNaCl）条件下，用非离子去污剂Triton X-100破坏纯化的VSV制剂。在低盐存在下产生的可沉淀核心颗粒仅含有三种主要病毒蛋白，并且在RNA的转录中具有活性。这些产物在大小分布、3 ′-聚腺苷酸序列的存在以及甲基化和封闭的5 ′-末端的存在方面与真实的vitroVSV mRNA种类相同。在高盐条件下破坏病毒体产生仅含有一种病毒蛋白的可沉淀核心，所述病毒蛋白仅当与病毒蛋白的相应上清液级分混合时才能转录RNA。通过这种重构产生的RNA产物也无法通过相同的标准与真实的VSV mRNA区分开。尝试甲基化外源VSV mRNA或合成的"帽"结构的分馏病毒粒子组件是不成功的。它的结论是，在VSV转录，阻断，甲基化，和聚腺苷酸化活动都是转录依赖性的，可以介导的最低限度的三个病毒蛋白。
Purified VSV preparations were disrupted by the nonionic detergent Triton X-100 in the presence of either low (0.4MNaCl) or high (0.8MNaCl) salt conditions. A sedimentable core particle produced in the presence of low salt contained only three of the principal viral proteins and was active in the transcription of RNA. These products were identical to authenticin vitroVSV mRNA species in their size distribution, the presence of 3′-polyadenylate sequences, and the presence of methylated and blocked 5′-termini. Disruption of virions in high salt conditions produced a sedimentable core containing only one viral protein which could transcribe RNA only when mixed with the corresponding supernatant fraction of viral proteins. The RNA products produced by such reconstitution were also indistinguishable from authentic VSV mRNAs by the same criteria. Attempts to methylate exogenous VSV mRNA or synthetic “cap” structures by fractionated virion components were unsuccessful. It is concluded that during VSV transcription, the blocking, methylating, and polyadenylating activities are all transcription dependent, and can be mediated by a minimum of three viral proteins.