DDX1 Promotes Proliferation of the JC Virus through Transactivation of Its Promoter
DDX1 Promotes Proliferation of the JC Virus through Transactivation of Its Promoter
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DOI：
10.1111/j.1348-0421.2007.tb03907.x
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发表时间：
2007-03
影响因子：
2.6
通讯作者：
Y. Sunden;S. Semba;Tadaki Suzuki;Y. Okada;Y. Orba;K. Nagashima;T. Umemura;H. Sawa
中科院分区：
文献类型：
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作者：
Y. Sunden;S. Semba;Tadaki Suzuki;Y. Okada;Y. Orba;K. Nagashima;T. Umemura;H. Sawa
最近，我们证明了DEAD盒蛋白1（DDX 1），一种RNA解旋酶，和切割刺激因子（CstF）形成一种复合物，与JC病毒转录控制区（JCV-TCR）结合。在这里，我们检查了DDX 1的功能，它在JCV易感细胞系IMR-32中的表达水平比在非易感细胞系中高得多。DDX 1对JCV的复制效率没有影响，但过表达DDX 1显著增加了JCV启动子的反式激活。此外，DDX 1增强了JCV感染细胞中JCV蛋白的表达，并且使用小干扰（si）RNA敲低DDX 1抑制了JCV蛋白的表达。我们的研究结果清楚地表明，DDX 1通过转录激活调节JCV在体外的增殖。
Recently, we demonstrated that the DEAD box protein 1 (DDX1), an RNA helicase, and the cleavage stimulation factor (CstF) form a complex that binds to the JC virus transcriptional control region (JCV‐TCR). Here, we examined the function of DDX1, which is expressed at much higher levels in the JCV‐susceptible cell line IMR‐32 than in non‐susceptible cell lines. DDX1 had no effect on the replication efficiency of JCV, but over expression of DDX1 significantly increased transactivation of the JCV promoter. Furthermore, DDX1 enhanced the expression of JCV proteins in JCV infected cells, and knockdown of DDX1 using small interfering (si) RNA suppressed the expression of JCV proteins. Our results clearly demonstrate that DDX1 regulates proliferation of JCV in vitro through transcriptional activation.