Site-Selective Labeling of Native Proteins by a Multicomponent Approach
Site-Selective Labeling of Native Proteins by a Multicomponent Approach
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DOI：
10.1002/chem.201605938
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发表时间：
2017-03-01
影响因子：
4.3
通讯作者：
Rai, Vishal
中科院分区：
文献类型：
--
作者：
Chilamari, Maheshwerreddy;Purushottam, Landa;Rai, Vishal
Chemical functionalization of proteins is an indispensable tool. Yet, selective labeling of native proteins has been an arduous task. The limited success of chemical methods allows N-terminus protein labeling, but the examples with side-chain residues are rare. Herein, we surpass this challenge through a multicomponent transformation that operates under physiological conditions in the presence of a protein, aldehyde, acetylene, and Cu-ligand complex. The methodology results in the labeling of a single lysine residue in nine distinct proteins.
