Molecular characterization of CD9 and CD63, two tetraspanin family members expressed in trout B lymphocytes
Molecular characterization of CD9 and CD63, two tetraspanin family members expressed in trout B lymphocytes
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DOI：
10.1016/j.dci.2015.03.002
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发表时间：
2015-07-01
影响因子：
2.9
通讯作者：
Tafalla, Carolina
中科院分区：
文献类型：
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作者：
Castro, Rosario;Abos, Beatriz;Tafalla, Carolina
四跨膜蛋白是一个膜组织蛋白家族，其特征在于存在四个高度保守的跨膜区域，介导多种生理功能。在目前的研究中，我们已经确定了两个新的tetraspanin成员虹鳟鱼（虹鳟鱼），同源哺乳动物的CD9和CD63。这两个基因在肌肉、皮肤、鳃、后肠、性腺、肝、脾、头肾、胸腺和外周血白细胞中表达。在整个生命周期的早期阶段，CD9 mRNA水平显着增加后，第一次喂养，而CD63转录保持不变，在所有的发育阶段分析。在实验浴感染病毒性出血性败血症病毒（VHSV），CD9转录下调鳃，而CD63 mRNA水平下调头肾。相反，当病毒被腹膜内注射时，在感染后几天，两种基因的转录在腹膜细胞中显著上调。此外，这两个基因在注射VHSV DNA疫苗的鳟鱼肌肉中转录上调。为了深入了解这些四跨膜蛋白与B细胞活性的关系，我们确定了它们在不同来源的幼稚IgM（+）群体中的组成型表达，并观察到这两种分子在不同组织中被IgM（+）细胞转录。此外，CD9转录显着下调脾IgM（+）细胞在体外VHSV暴露的反应。我们的研究结果提供了这些四跨膜蛋白的硬骨鱼B细胞和抗病毒免疫的潜在作用的见解。(C)2015爱思唯尔有限公司版权所有。
Tetraspanins are a family of membrane-organizing proteins, characterized by the presence of four highly conserved transmembrane regions that mediate diverse physiological functions. In the current study, we have identified two novel tetraspanin members in rainbow trout (Oncorhynchus mykiss), homologs to mammalian CD9 and CD63. Both genes were expressed in muscle, skin, gills, hindgut, gonad, liver, spleen, head kidney, thymus and peripheral blood leukocytes. Throughout the early life cycle stages, CD9 mRNA levels significantly increased after first feeding, whereas CD63 transcription remained constant during all the developmental stages analyzed. In response to an experimental bath infection with viral hemorrhagic septicemia virus (VHSV), CD9 transcription was down-regulated in the gills, while CD63 mRNA levels were down-regulated in the head kidney. Instead, when the virus was intraperitoneally injected, the transcription of both genes was significantly up-regulated in peritoneal cells at several days post-infection. Additionally, both genes were transcriptionally up-regulated in the muscle of trout injected with a VHSV DNA vaccine. To gain insight on the relation of these tetraspanins with B cell activity we determined their constitutive expression in naive IgM(+) populations from different sources and observed that both molecules were being transcribed by IgM(+) cells in different tissues. Furthermore, CD9 transcription was significantly down-regulated in splenic IgM(+) cells in response to in vitro VHSV exposure. Our results provide insights on the potential role of these tetraspanins on teleost B cell and antiviral immunity. (C) 2015 Elsevier Ltd. All rights reserved.