Pseudomonas aeruginosa ExoU, a toxin trnasported by the type III secretion system, kills Saccharomyces cerevisiae
Pseudomonas aeruginosa ExoU, a toxin trnasported by the type III secretion system, kills Saccharomyces cerevisiae
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DOI：
10.1128/iai.71.7.4144-4150.2003
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发表时间：
2003-07-01
影响因子：
3.1
通讯作者：
Hauser, AR
中科院分区：
文献类型：
--
作者：
Rabin, SDP;Hauser, AR
ExoU是一种由铜绿假单胞菌III型分泌系统转运的蛋白，是一种重要的细胞毒素，但其作用机制尚不清楚。在这里，我们证明了ExoU在细胞内的表达对酿酒酵母具有细胞毒性。此外，内部氨基和羧基末端的缺失证实了以前被证明是杀死哺乳动物细胞所必需的ExoU区域也是杀死酵母细胞所必需的。这些发现表明，酿酒酵母是研究ExoU的有用模式生物。
ExoU, a protein transported by the type III secretion system of Pseudomonas aeruginosa, is an important cytotoxin, though its mechanism of action is unclear. Here we show that the intracellular expression of ExoU is cytotoxic to Saccharomyces cerevisiae. Furthermore, internal amino- and carboxyl-terminal deletions confirmed that regions of ExoU previously shown to be essential for killing mammalian cells were also required for killing yeast cells. These findings indicate that S. cerevisiae is a useful model organism for the study of ExoU.