Salmonella enterica serovar Choleraesuis infection of the porcine jejunal IL-1β Peyer's patch rapidly induces and IL-8 expression
Salmonella enterica serovar Choleraesuis infection of the porcine jejunal IL-1β Peyer's patch rapidly induces and IL-8 expression
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DOI：
10.1016/j.vetimm.2005.06.016
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发表时间：
2006-01-15
影响因子：
1.8
通讯作者：
Murtaugh, MP
中科院分区：
文献类型：
--
作者：
Hyland, KA;Brown, DR;Murtaugh, MP
猪霍乱沙门氏菌是猪的肠道病原体，可引起败血症、小肠结肠炎、肺炎和肝炎。据推测，沙门氏菌感染部位的初始分子事件对于先天性和适应性免疫反应的启动至关重要；然而，猪肠道组织引起的急性免疫反应尚不清楚。为了满足这一需求，我们使用猪的空肠集合淋巴结（JPP）粘膜外植体来检查受控条件下沙门氏菌诱导的免疫反应，并克服整体动物方法的局限性。安装在尤斯室中的 JPP 外植体在 2 小时内保持正常的组织学结构，并在 2.5 小时内保持稳定的短路电流和电导。在体外管腔暴露于猪霍乱沙门氏菌后，JPP 的反应是 IL-1β 和 IL-8 的 mRNA 表达增加，但 TNFα 的 mRNA 表达没有增加。 IL-1β和IL-8表达的增加依赖于有效的沙门氏菌粘附和内化，而突变型沙门氏菌不诱导炎症细胞因子表达。一些实验中存在的共生肠道细菌也不会诱导炎症细胞因子的表达。这些发现表明，派尔氏斑吸收沙门氏菌对于诱导涉及 IL-1β 和 IL-8 表达的先天反应非常重要，并且离体肠道免疫组织外植体提供了完整的组织模型，将有助于研究猪的粘膜免疫。 (C) 2005 Elsevier B.V. 保留所有权利。
Salmonella enterica serovar Choleraesuis is an enteric pathogen of swine, producing septicemia, enterocolitis, pneumonia, and hepatitis. The initial molecular events at the site of Salmonella infection are hypothesized to be critical in the initiation of innate and adaptive immune responses; however, the acute immune response elicited by porcine intestinal tissues is not well understood. To address this need, we employed explants of jejunal Peyer's patch (JPP) mucosa from pigs to examine Salmonella-induced immune responses under controlled conditions as well as to overcome limitations of whole animal approaches. JPP explants mounted in Ussing chambers maintained normal histological structure for 2 h and stable short-circuit current and electrical conductance for 2.5 h. After ex vivo luminal exposure to Salmonella serovar Choleraesuis, JPP responded with an increase in mRNA expression of IL-1 beta and IL-8, but not TNF alpha. Increased IL-1 beta and IL-8 expression were dependent on efficient Salmonella adhesion and internalization, whereas mutant Salmonella did not induce inflammatory cytokine expression. Commensal enteric bacteria, present in some experiments, also did not induce inflammatory cytokine expression. These findings indicate that Salmonella uptake by Peyer's patch is important in the induction of an innate response involving expression of IL-1 beta and IL-8, and that ex vivo intestinal immune tissue explants provide an intact tissue model that will facilitate investigation of mucosal immunity in swine. (C) 2005 Elsevier B.V. All rights reserved.