Different regulatory regions are required for the vernalization-induced repression of FLOWERING LOCUS C and for the epigenetic maintenance of repression
Different regulatory regions are required for the vernalization-induced repression of FLOWERING LOCUS C and for the epigenetic maintenance of repression
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DOI：
10.1105/tpc.004564
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发表时间：
2002-10-01
期刊：
影响因子：
11.6
通讯作者：
Peacock, WJ
中科院分区：
文献类型：
--
作者：
Sheldon, CC;Conn, AB;Peacock, WJ
春化作用，即通过长时间的低温促进开花，导致花阻遏物FLOWERLOCUS C（FLC）的阻遏和早花。这种抑制具有表观遗传事件的标志：在许多细胞分裂周期中维持低表达状态，但在后代中表达被去抑制。我们表明，两个阶段的FLC春化反应，FLC的镇压和维持镇压在正常温度下的生长过程中春化后，是通过不同区域的FLC基因介导的。启动子区和基因内区都是应答所必需的。我们还确定了一个75 bp的区域，在FLC启动子，除了基因内序列，是所需的nonvernalized植物中的表达。
Vernalization, the promotion of flowering by a prolonged period of low temperature, results in repression of the floral repressor FLOWERING LOCUS C (FLC) and in early flowering. This repression bears the hallmark of an epigenetic event: the low expression state is maintained over many cell division cycles, but expression is derepressed in progeny. We show that the two stages of the response of FLC to vernalization, the repression of FLC and the maintenance of the repression during growth at normal temperatures after vernalization, are mediated through different regions of the FLC gene. Both promoter and intragenic regions are required for the responses. We also identify a 75-bp region in the FLC promoter that, in addition to intragenic sequences, is required for expression in nonvernalized plants.