Effects of inhibiting phenolic biosynthesis on penetration resistance of barley isolines containing seven powdery mildew resistance genes or alleles
Effects of inhibiting phenolic biosynthesis on penetration resistance of barley isolines containing seven powdery mildew resistance genes or alleles
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DOI：
10.1016/s0885-5765(02)90415-7
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发表时间：
2002-07-01
影响因子：
2.7
通讯作者：
Zeyen, RJ
中科院分区：
文献类型：
--
作者：
Kruger, WM;Carver, TLW;Zeyen, RJ
大麦叶表皮细胞能有效抵抗白粉菌Blumeria graminis f.sp.萌发分生孢子的侵染。霍尔迪(BGH)。对适当的特殊形式的穿透阻力(f.sp.)BG的抗性对苯丙烷类化合物的生物合成的抑制很敏感，但其与已知的小种特异的白粉病抗性基因(R基因)和等位基因相关的遗传基础令人困惑。本研究探讨了苯丙烷类化合物抑制对R基因或等位基因近等基因大麦品系穿透抗性的影响。使用了Pallas(P)近等基因大麦的7个成员。苯丙氨酸解氨酶(PAL)的抑制剂α-氨基氧基-β-苯丙酸(AOPP)和肉桂醇脱氢酶的抑制剂([2-羟基苯氨基]亚氨基乙酸二甲酯(OH-PAS))对苯丙烷类化合物的生物合成有抑制作用。苯丙醇抑制减少了BGH生殖管接触部位的局部自发荧光，并显著增加了真菌的穿透性(P<；0.05)。亲本Pallas和含有Mla1(P01)、Mla12(P10)、Mla13(P11)、MLG(P21)、MLK(P17)和MLP(P19)R基因的近等基因系也是如此。这些R基因对苯丙烷类化合物的穿透抗性敏感性没有明显影响。然而，在具有非特异隐性等位基因mlo5的P22中，AOPP或OH-PAS处理对穿透抗性没有影响，尽管这两种抑制剂都降低了真菌胚管接触部位的局部自发荧光的频率和强度。除P22外，所有品系对苯丙烷类抑制剂的穿透抗性都具有相同的敏感性，这似乎是Pallas遗传背景的特性，而不是与小种特有的R基因或其等位基因相关。因此，mlo5的穿透抗性具有一个共同的生理现象，即对苯丙烷类化合物的抑制不敏感，大麦、小麦和燕麦在受到不适当的Formae Speciales(ff.Spp.)BG的。(C)2002爱思唯尔科学有限公司。保留所有权利。
Barley leaf epidermal cells actively resist penetration by germinated conidia of the powdery mildew fungus Blumeria graminis f.sp. hordei (Bgh). Penetration resistance to the appropriate forma specialis (f. sp.) of Bg is sensitive to inhibition of phenylpropanoid biosynthesis but its genetic basis relative to known race-specific powdery mildew resistance genes (R-genes) and alleles is confusing. In this study, the effects of phenylpropanoid inhibition on penetration resistance in near-isogenic barley lines with genetically defined R-genes or alleles were investigated. Seven members of the Pallas (P) near-isogenic barley set were used. Phenylpropanoid biosynthesis inhibition was accomplished using alpha-Aminooxy-beta-phenylpropionic acid (AOPP), an inhibitor of phenylalanine ammonia lyase (PAL), and ([2-hydroxyphenyl amino] sulfinyl) acetic acid, 1,1-dimethyl ester (OH-PAS) an inhibitor of cinnamyl-alcohol dehydrogenase. Phenylpropanoid inhibition decreased localized autofluorescence at Bgh germ tube contact sites and significantly (P < 0.05) increased fungal penetration. This was true of the parent Pallas and the near-isogenic lines containing Mla1 (P01), Mla12 (P10), Mla13 (P11), Mlg (P21), Mlk (P17) and Mlp (P19) R-genes. These R-genes had no apparent effect on phenylpropanoid sensitivity of penetration resistance. However, in P22, with the race non-specific recessive allele mlo5, penetration resistance was unaffected by either AOPP or OH-PAS treatments, even though both inhibitors reduced the frequency and intensity of localized autofluorescence at fungal germ tube contact sites. The sensitivity of penetration resistance to the phenylpropanoid inhibitors was shared by all isolines, except P22, and appeared to be a property of the Pallas genetic background rather than being associated with race-specific R-genes or their alleles. Thus mlo5 penetration resistance shares a physiological phenomena i.e. insensitivity to phenylpropanoid inhibition, with "non-host" penetration resistance exhibited by barley, wheat and oat when they are attacked by inappropriate formae speciales (ff. spp.) of Bg. (C) 2002 Elsevier Science Ltd. All rights reserved.