Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis
Purification and characterization of a novel type of fimbriae from the oral anaerobe Bacteroides gingivalis
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口腔厌氧菌牙龈拟杆菌新型菌毛的纯化和表征
DOI：
10.1128/jb.160.3.949-957.1984
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发表时间：
1984
影响因子：
3.2
通讯作者：
T. Suzuki
中科院分区：
文献类型：
--
作者：
F. Yoshimura;K. Takahashi;Y. Nodasaka;T. Suzuki
分别从牙龈拟杆菌381的细菌洗涤液和细胞溶解产物部分中将菌毛及其组成蛋白（菌毛蛋白）纯化至同质。负染法观察菌毛为卷曲的单链丝状体，直径约为2.5 μ m。5nm。菌毛蛋白的表观分子量为43，000。菌毛对70 ℃十二烷基硫酸钠变性有抵抗力。需要在十二烷基硫酸钠中在100 ° C下加热以使它们完全解离为菌毛蛋白的单体。不同的抗原决定簇似乎暴露在菌毛和十二烷基硫酸钠变性菌毛蛋白的表面上。纯化的菌毛未显示血凝活性或血凝抑制活性，尽管根据间接证据推断菌毛与生物体的血凝活性相关。然而，在培养上清液中检测到血凝素活性，这一观察结果表明不同类型的菌毛或凝集素样蛋白可能在B中起血凝素的作用。牙龈炎
Fimbriae and their constituent protein (fimbrilin) were purified to homogeneity from the bacterial wash fluid and cell lysate fraction, respectively, of Bacteroides gingivalis 381. Fimbriae, observed by negative staining, were curly, single-stranded filaments with a diameter of ca. 5 nm. The apparent molecular weight of the fimbrilin was 43,000. Fimbriae were resistant to sodium dodecyl sulfate denaturation at 70 degrees C. Heating at 100 degrees C in sodium dodecyl sulfate was needed to completely dissociate them to monomers of fimbrilin. Different sets of antigenic determinants seemed to be exposed on the surfaces of fimbriae and sodium dodecyl sulfate-denatured fimbrilin. Purified fimbriae did not show either hemagglutinating activity or hemagglutination inhibitory activity, although it has been inferred on the basis of circumstantial evidence that fimbriae are correlated to hemagglutinating activity of the organism. Hemagglutinin activity, however, was detected in culture supernatant, and this observation suggests that fimbriae of a different type or a lectin-like protein may be acting as hemagglutinin in B. gingivalis.
DOI：
--
发表时间：
1981
期刊：
Annales de microbiologie
影响因子：
--
作者：
Mouton,C;Hammond,PG;Slots,J;Reed,MJ;Genco,RJ
通讯作者：
Genco,RJ
口腔中间拟杆菌的血清学研究。
影响因子：
3.1
作者：
Nakazawa,F;Zambon,JJ;Reynolds,HS;Genco,RJ
通讯作者：
Genco,RJ