The roles of lipid in anti-biofilm efficacy of lipid-polymer hybrid nanoparticles encapsulating antibiotics
The roles of lipid in anti-biofilm efficacy of lipid-polymer hybrid nanoparticles encapsulating antibiotics
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DOI：
10.1016/j.colsurfa.2011.08.035
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发表时间：
2011-09-20
影响因子：
5.2
通讯作者：
Hadinoto, Kunn
中科院分区：
文献类型：
--
作者：
Cheow, Wean Sin;Chang, Matthew Wook;Hadinoto, Kunn
由于其物理稳健性，脂质-聚合物混合纳米颗粒被开发为潜在地替代脂质体，其对生物膜细胞具有高亲和力，作为肺生物膜感染治疗中的抗生素递送载体。为此目的，在体外针对铜绿假单胞菌生物膜细胞检查了载药的混合纳米颗粒的抗菌活性，用于纳米颗粒的两种药物剂型-水性悬浮液和干粉。将混合纳米颗粒的抗生物膜功效与聚合物纳米颗粒的抗生物膜功效进行比较，以阐明脂质包含物（如果有的话）对生物膜根除的影响。聚（乳酸-羟基乙酸共聚物）、磷脂酰胆碱和左氧氟沙星分别用作聚合物、脂质和抗生素模型。与聚合物纳米颗粒相比，杂化纳米颗粒的尺寸相似，但更稳定。混合纳米颗粒中脂质涂层的存在导致抗生素释放速率较慢，但它不会改善其生物膜亲和力。混合纳米颗粒对生物膜细胞表现出更高的抗菌功效，但对从生物膜基质脱落的嗜酸性细胞没有。聚合物纳米颗粒和混合纳米颗粒之间的抗生素活性、释放速率和生物膜细胞脱离的变化已经被排除为有助于更高的抗生物膜功效的因素。除了抗生素敏感性测试之外，还需要进行后续研究，以确定混合纳米颗粒增强抗生物膜的机制。(C)2011爱思唯尔有限公司版权所有。
Owing to their physical robustness, lipid-polymer hybrid nanoparticles are developed to potentially replace liposomes, which possess high affinity towards biofilm cells, as antibiotic delivery vehicles in lung biofilm infection therapy. Towards this aim, antibacterial activity of antibiotic-loaded hybrid nanoparticles is examined in vitro against Pseudomonas aeruginosa biofilm cells for two pharmaceutical dosage forms of the nanoparticles - aqueous suspension and dry-powders. Anti-biofilm efficacy of the hybrid nanoparticles is compared to that of polymeric nanoparticles to elucidate the impact of lipid inclusion, if any, on biofilm eradication. Poly (lactic-co-glycolic acid), phosphatidylcholine, and levofloxacin are employed as the polymer, lipid, and antibiotic models, respectively. Compared to polymeric nanoparticles, hybrid nanoparticles are similar in size but more stable. The presence of lipid coat in the hybrid nanoparticles results in slower antibiotic release rates, but it does not improve their biofilm affinity. Hybrid nanoparticles exhibit higher antibacterial efficacy against biofilm cells, but not against planktonic cells shed from the biofilm matrix. Variations in the antibiotic activity, release rate, and biofilm cell detachment between the polymeric and hybrid nanoparticles have been ruled out as factors contributing to the higher anti-biofilm efficacy. Follow-up studies beyond antibiotic susceptibility testing are needed to identify the mechanisms for the anti-biofilm enhancement by hybrid nanoparticles. (C) 2011 Elsevier B.V. All rights reserved.