ASSESSMENT OF MICROCYSTIS BLOOM TOXICITY ASSOCIATED WITH WILDLIFE MORTALITY IN THE KRUGER NATIONAL PARK, SOUTH AFRICA
ASSESSMENT OF MICROCYSTIS BLOOM TOXICITY ASSOCIATED WITH WILDLIFE MORTALITY IN THE KRUGER NATIONAL PARK, SOUTH AFRICA
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南非克鲁格国家公园微囊藻华毒性与野生动物死亡率的评估
DOI：
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发表时间：
2010
影响因子：
1.3
通讯作者：
Dharmarai Naicker
中科院分区：
文献类型：
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作者：
M. Masango;J. Myburgh;L. Labuschagne;D. Govender;R. Bengis;Dharmarai Naicker
根据之前的尸检结果，克鲁格国家公园（KNP）建筑蓄水池中的微囊藻水华已被确定为野生动物死亡的原因。为了应对2007年野生动物死亡率，在2007年2月和2007年7月期间从KNP东南部的四个大坝（Nhlanganzwani，Mpanamana，Makhohlola和Sunset）收集了含有藻华物质的水样，作为后续调查的一部分。采用酶联免疫吸附试验（ELISA）、蛋白磷酸酶抑制试验（PPI）、小鼠生物测定和非洲尖齿鲶（Clarias gariepinus）原代肝细胞测定了微囊藻水华的毒性。ELISA和PPI分析均表明，2007年2月从Nhlanganzwani大坝采集的水样以及2007年6月从Nhlanganzwani和Sunset大坝采集的水样都是有毒的。这些大坝，展示有毒微囊藻水华，也与野生动物的死亡率。从Nhlanganzwani大坝（2007年2月）的水样品腹腔注射小鼠诱导肝毒性和死亡率在1小时内。从尖齿鲶鱼暴露于这些大坝的样品的原代肝细胞给了类似的结果。本实验室的调查和结果有力地证明了有毒微囊藻水华是野生动物死亡的原因。富营养化和水华的形成似乎是特定水坝中大量河马（Hippopotamus amphibus）的后果。
Based on previous necropsy results, Microcystis blooms in constructed water impoundments in the Kruger National Park (KNP) have been identified as a cause of wildlife mortality. In response to wildlife mortality during 2007, water samples, containing algal bloom material, were collected during February 2007 and July 2007 from four dams (Nhlanganzwani, Mpanamana, Makhohlola, and Sunset) in the southeastern part of the KNP as part of the follow-up investigation. The toxicity of the Microcystis blooms was determined using the enzyme-linked immunosorbent assay (ELISA), protein phosphatase inhibition (PPI) assay, mouse bioassay, and African sharptooth catfish (Clarias gariepinus) primary hepatocytes. Both the ELISA and PPI assays indicated that the water sample collected during February 2007 from the Nhlanganzwani Dam, and samples collected from the Nhlanganzwani and Sunset dams in June 2007, were toxic. These dams, exhibiting the toxic Microcystis blooms, were also associated with the wildlife mortality. Mice injected intraperitoneally with water samples from Nhlanganzwani Dam (February 2007) induced hepatotoxicity and mortality within 1 hr. Primary hepatocytes from the sharptooth catfish exposed to samples from these dams gave similar results. This laboratory investigation and results strongly incriminate the toxic Microcystis blooms as the cause of the wildlife mortality. Eutrophication and bloom formation appear to have been the consequence of the high numbers of hippopotami (Hippopotamus amphibius) in specific dams.
影响因子：
11.4
作者：
Fischer, WJ;Garthwaite, I;Dietrich, DR
通讯作者：
Dietrich, DR