Cholesteryl hemisuccinate exhibits pH sensitive polymorphic phase behavior
Cholesteryl hemisuccinate exhibits pH sensitive polymorphic phase behavior
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DOI：
10.1016/s0005-2736(99)00186-8
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发表时间：
2000-01-15
影响因子：
3.4
通讯作者：
Cullis, PR
中科院分区：
文献类型：
--
作者：
Hafez, IM;Cullis, PR
半琥珀酸胆固醇酯 (CHEMS) 是一种酸性胆固醇酯，可在碱性和中性水介质中自组装成双层，通常与二油酰磷脂酰乙醇胺 (DOPE) 混合使用，形成“pH 敏感”融合囊泡。我们在此表明 CHEMS 本身表现出 pH 敏感多态性。从由 CHEMS 组成的大单层囊泡 (LW) 的融合特性和采用冷冻断裂电子显微镜的直接可视化可以明显看出这一点。 pH 值低于 4.3 时，由 CHEMS 组成的 LUV 发生融合（通过脂质混合测定监测），冷冻断裂电子显微照片揭示了与脂质平行的 H 相特征条纹特征。这表明 CHEMS 的 pH 依赖性相偏好有助于由 CHEMS 和 DOPE 混合物组成的 LUV 的 pH 敏感性。 (C) 2000 Elsevier Science B.V. 保留所有权利。
Cholesteryl hemisuccinate (CHEMS) is an acidic cholesterol ester that self-assembles into bilayers in alkaline and neutral aqueous media and is commonly employed in mixtures with dioleoylphosphatidylethanolamine (DOPE) to form 'pH sensitive' fusogenic vesicles. We show here that CHEMS itself exhibits pH sensitive polymorphism. This is evident from the fusogenic properties of large unilamellar vesicles (LW) composed of CHEMS and direct visualization employing freeze-fracture electron microscopy. Below pH 4.3, LUV composed of CHEMS undergo fusion as monitored by lipid mixing assays and freeze-fracture electron micrographs reveal the characteristic striated signature of H-parallel to phase lipid. It is suggested that the pH dependent phase preferences of CHEMS contribute to the pH sensitivity of LUV composed of mixtures of CHEMS and DOPE. (C) 2000 Elsevier Science B.V. All rights reserved.